概述

  • 产品名称Anti-HA tag抗体
    参阅全部 HA tag 一抗
  • 描述
    兔多克隆抗体to HA tag
  • 经测试应用适用于: WB, ChIP, ICC/IFmore details
  • 种属反应性
    Not appplicable.
  • 免疫原

    Synthetic peptide:

    CYPYDVPDYAYPYDVPDYA

    encoding HA tag conjugated to KLH

  • 阳性对照
    • HA-tagged recombinant protein bacterial lysate.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 纯度Ammonium Sulphate Precipitation
  • 纯化说明This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab71113 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/50 - 1/100. Detects a band of approximately 50 kDa.
ChIP Use at an assay dependent concentration.
ICC/IF 1/10 - 1/50.

靶标

  • 相关性Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
  • 别名
    • HA epitope tag antibody
    • HA1 antibody
    • HA2 antibody
    • hemagglutinin antibody
    • Hemagglutinin HA1 chain antibody
    • Hemagglutinin HA2 chain antibody
    see all

Anti-HA tag antibody 图像

  • All lanes : Anti-HA tag antibody (ab71113)

    Lane 1 : HA-tagged recombinant protein bacterial lysate
    Lane 2 : HA-tagged recombinant protein bacterial lysate

    Lysates/proteins at 12.5 µg per lane.


    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
  • Immunofluorescent staining of MRC-5 fibroblasts transiently transfected with either wild-type (wt) or a GTPase-deficient dynamin-2 mutant (K44A) with a hemagglutinin (HA) tag using ab71113.

  • ChIP was performed with 35S:HATCL1 plants using anti-HA antibodies. Rabbit preimmune serum was used as a mock control. Primer sets specific for the first intron or the 3-UTR region of GL1 were used in PCR reactions. ACTIN2 was used as a control.

Anti-HA tag antibody (ab71113)参考文献

This product has been referenced in:
  • Duval N  et al. Msx1 and Msx2 act as essential activators of Atoh1 expression in the murine spinal cord. Development 141:1726-36 (2014). Read more (PubMed: 24715462) »

See 1 Publication for this product

Product Wall

Thank you for your enquiry, I am pleased to help.

From what I understand here, the customer would liketo detect the HA tag on their recombinant protein expressed in cell culture. This is Immunocytochemistry (ICC). I have reviewed the selecti...

Read More

Thank you for contacting us.

I am sorry that this antibody did not perform as stated on the datasheet. I have asked our accounting department to issue a credit note for you, which can be redeemed against the invoice of a future order by pass...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"