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Our Abpromise guarantee covers the use of ab71113 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/50 - 1/100. Detects a band of approximately 50 kDa.|
|ChIP||Use at an assay dependent concentration.|
|ICC/IF||1/10 - 1/50.|
Immunofluorescent staining of MRC-5 fibroblasts transiently transfected with either wild-type (wt) or a GTPase-deficient dynamin-2 mutant (K44A) with a hemagglutinin (HA) tag using ab71113.
ChIP was performed with 35S:HATCL1 plants using anti-HA antibodies. Rabbit preimmune serum was used as a mock control. Primer sets specific for the first intron or the 3-UTR region of GL1 were used in PCR reactions. ACTIN2 was used as a control.