概述

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab13834 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
  • 应用说明
    WB: 1/500. Can be blocked with HA tag peptide.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性
      Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
    • 别名
      • HA epitope tag antibody
      • HA1 antibody
      • HA2 antibody
      • hemagglutinin antibody
      • Hemagglutinin HA1 chain antibody
      • Hemagglutinin HA2 chain antibody
      see all

    图片

    • All lanes : Anti-HA tag antibody (ab13834) at 1/500 dilution

      Lane 1 : HEK293 (Human) Whole Cell Lysate - expressing HA tag protein (ab20896)
      Lane 2 : HEK293 (Human) Whole Cell Lysate - expressing HA tag protein (ab20896) with HA tag peptide (ab13835) at 1 µg/ml

      Lysates/proteins at 10 µg per lane.

      Secondary
      Alexa Fluor 680 Goat-anti-Rabbit IgG at 1/5000 dilution

      Performed under reducing conditions.

      Observed band size : 55 kDa (why is the actual band size different from the predicted?)

    实验方案

    文献

    This product has been referenced in:
    • Myers SA  et al. SOX2 O-GlcNAcylation alters its protein-protein interactions and genomic occupancy to modulate gene expression in pluripotent cells. Elife 5:e10647 (2016). Read more (PubMed: 26949256) »
    • Chen X  et al. Deficiency or inhibition of lysophosphatidic acid receptor 1 protects against hyperoxia-induced lung injury in neonatal rats. Acta Physiol (Oxf) 216:358-75 (2016). Read more (PubMed: 26495902) »

    See all 6 Publications for this product

    客户评价及客户问答

    Application
    IHC - Wholemount
    Sample
    Mouse Tissue (Brain)
    Specification
    Brain
    Username

    Prof. Vidita Vaidya

    Verified customer

    提交于 Jul 23 2014

    Thank you for your enquiry, I am pleased to help.

    From what I understand here, the customer would liketo detect the HA tag on their recombinant protein expressed in cell culture. This is Immunocytochemistry (ICC). I have reviewed the selecti...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HeLa cells)
    Loading amount
    40 µg
    Specification
    HeLa cells
    Gel Running Conditions
    Reduced Denaturing (10% Bis-Tris)
    Blocking step
    LI-COR® Odyssey® Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C
    Username

    Dr. Svetlana Khoronenkova

    Verified customer

    提交于 Aug 17 2010

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Human glioma cells)
    Loading amount
    250000 cells
    Specification
    Human glioma cells
    Treatment
    Transfected with lipofectamine
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    提交于 Sep 04 2007

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    注册