Anti-HA tag抗体[16B12] (DyLight® 650) (ab117515)

概述

  • 产品名称
    Anti-HA tag抗体[16B12] (DyLight® 650)
    参阅全部 HA tag 一抗
  • 描述
    小鼠单克隆抗体[16B12] to HA tag (DyLight® 650)
  • 偶联物
    DyLight® 650. Ex: 654nm, Em: 673nm
  • 特异性
    ab117515 recognizes the Influenza hemagglutinin epitope (YPYDVPDYA).
  • 经测试应用
    适用于: ICC/IF, Flow Cytmore details
  • 免疫原

    Synthetic peptide

    YPYDVPDYA

    corresponding to HA tag.

  • 常规说明

    Stable for 1yr.

    Conjugated to Dylight® 650 (ratio 1.3:): Visible absorption maxima 652 nm; Emission maximum 672 nm.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C.
  • 存储溶液
    pH: 8.20
    Constituent: 99% Tris buffered saline
  • Concentration information loading...
  • 纯度
    Protein G purified
  • 克隆
    单克隆
  • 克隆编号
    16B12
  • 同种型
    IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab117515 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/500 - 1/5000.
Flow Cyt Use at an assay dependent concentration.

靶标

  • 相关性
    Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
  • 别名
    • HA epitope tag antibody
    • HA1 antibody
    • HA2 antibody
    • hemagglutinin antibody
    • Hemagglutinin HA1 chain antibody
    • Hemagglutinin HA2 chain antibody
    see all

图片

  • ab117515 staining 293T cells expressing a HA-tagged protein (intracellular) by Flow Cytometry. Cells were permeabilized and fixed. The sample was incubated with the primary antibody (1/200) for 30 minutes at 4°C.

    Gating Strategy: Gated on FSC-SSC to exclude debris/dead cells.

    Grey - unstained cells.

    Red - Wild type 293T, stained.

    Blue - 293T expressing HA-tagged protein, stained.

    See Abreview

文献

This product has been referenced in:
  • Greenwood EJ  et al. Temporal proteomic analysis of HIV infection reveals remodelling of the host phosphoproteome by lentiviral Vif variants. Elife 5:N/A (2016). Read more (PubMed: 27690223) »

See 1 Publication for this product

客户评价及客户问答

Application
Flow Cytometry
Fixation
BD Fix/Perm
Permeabilization
Yes - BD Perm/Wash
Sample
Human Cell (293T)
Specification
293T
Gating Strategy
Gated on FSC-SSC to exclude debris/dead cells
Preparation
Cell harvesting/tissue preparation method: WT and cells expressing an HA-tagged protein (intracellular)
Sample buffer: BD fix/perm and perm/wash
Username

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提交于 Mar 11 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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