Anti-HA tag抗体[16B12] (DyLight® 650) (ab117515)

概述

  • 产品名称Anti-HA tag抗体[16B12] (DyLight® 650)
    参阅全部 HA tag 一抗
  • 描述
    小鼠单克隆抗体[16B12] to HA tag (DyLight® 650)
  • 偶联物DyLight® 650. Ex: 654nm, Em: 673nm
  • 特异性ab117515 recognizes the Influenza hemagglutinin epitope (YPYDVPDYA).
  • 经测试应用适用于: ICC/IF, Flow Cytmore details
  • 免疫原

    Synthetic peptide

    YPYDVPDYA

    corresponding to HA tag.

  • 常规说明

    Stable for 1yr.

    Conjugated to Dylight® 650 (ratio 1.3:): Visible absorption maxima 652 nm; Emission maximum 672 nm.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C.
  • 存储溶液pH: 8.20
    Constituent: 99% Tris buffered saline
  • Concentration information loading...
  • 纯度Protein G purified
  • 克隆单克隆
  • 克隆编号16B12
  • 同种型IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab117515 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/500 - 1/5000.
Flow Cyt Use at an assay dependent concentration.

靶标

  • 相关性Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
  • 别名
    • HA epitope tag antibody
    • HA1 antibody
    • HA2 antibody
    • hemagglutinin antibody
    • Hemagglutinin HA1 chain antibody
    • Hemagglutinin HA2 chain antibody
    see all

Anti-HA tag antibody [16B12] (DyLight® 650) 图像

  • ab117515 staining 293T cells expressing a HA-tagged protein (intracellular) by Flow Cytometry. Cells were permeabilized and fixed. The sample was incubated with the primary antibody (1/200) for 30 minutes at 4°C.

    Gating Strategy: Gated on FSC-SSC to exclude debris/dead cells.

    Grey - unstained cells.

    Red - Wild type 293T, stained.

    Blue - 293T expressing HA-tagged protein, stained.

    See Abreview

Anti-HA tag antibody [16B12] (DyLight® 650) (ab117515)参考文献

ab117515 has not yet been referenced specifically in any publications.

Product Wall

Application Flow Cytometry
Fixation BD Fix/Perm
Permeabilization Yes - BD Perm/Wash
Sample Human Cell (293T)
Specification 293T
Gating Strategy Gated on FSC-SSC to exclude debris/dead cells
Preparation Cell harvesting/tissue preparation method: WT and cells expressing an HA-tagged protein (intracellular)
Sample buffer: BD fix/perm and perm/wash
Username

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Verified customer

提交于 Mar 11 2015

We would recommend dilution in TBS as this is the storage buffer the antibody ships with. After dilution we recommend long term storage at -20C. I have attached our Buffers and stock solutions pdf which includes TBS recipes. Your caluculations are c...

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Vielen Dank für das Ausfüllen des Fragenbogens.
Bitte bestätigen Sie mir, dass die Expression des HA-tags auf andere Weise nachgewiesen wurde.
Ich kann Ihnen dann gerne ein neues Röhrchen (auch von einem alternativen Antikörper) als Ersatz sc...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"