Anti-hCAP-D3抗体(ab70349)
Key features and details
- Rabbit polyclonal to hCAP-D3
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
概述
-
产品名称
Anti-hCAP-D3抗体
参阅全部 hCAP-D3 一抗 -
描述
兔多克隆抗体to hCAP-D3 -
宿主
Rabbit -
特异性
We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues. -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Human
预测可用于: Chimpanzee, Rhesus monkey, Gorilla -
免疫原
Synthetic peptide corresponding to a region between C terminal residues 1450-1507 of human hCAP-D3. (NP_056076.1)
-
阳性对照
- HeLa and 293T whole cell lysate
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
-
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab70349于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | ||
IP |
说明 |
---|
WB: 1/2000 - 1/10000. Detects a band of approximately 174 kDa (predicted molecular weight: 168 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
靶标
-
功能
Regulatory subunit of the condensin-2 complex, a complex which establishes mitotic chromosome architecture and is involved in physical rigidity of the chromatid axis. -
序列相似性
Contains 4 HEAT repeats. -
细胞定位
Nucleus. - Information by UniProt
-
数据库链接
- Entrez Gene: 23310 Human
- Omim: 609276 Human
- SwissProt: P42695 Human
- Unigene: 438550 Human
-
别名
- CAP D3 antibody
- CAPD 3 antibody
- CAPD3 antibody
see all
图片
-
All lanes:200µg Hela whole cell lysate and ab70349 at 1µg/ml. Lane 1: ab70349 at 3 µg/mg lysate for IP. Lane 2: IgG control.
-
All lanes : Anti-hCAP-D3 antibody (ab70349) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size: 168 kDa
Observed band size: 174 kDa why is the actual band size different from the predicted?
数据表及文件
-
SDS download
-
Datasheet download
文献 (9)
ab70349 被引用在 9 文献中.
- Choi EH et al. Meiosis-specific cohesin complexes display essential and distinct roles in mitotic embryonic stem cell chromosomes. Genome Biol 23:70 (2022). PubMed: 35241136
- Winans S et al. A point mutation in HIV-1 integrase redirects proviral integration into centromeric repeats. Nat Commun 13:1474 (2022). PubMed: 35304442
- Wallace HA et al. Condensin II subunit NCAPH2 associates with shelterin protein TRF1 and is required for telomere stability. J Cell Physiol N/A:N/A (2019). PubMed: 31026066
- Baergen AK et al. Diminished Condensin Gene Expression Drives Chromosome Instability That May Contribute to Colorectal Cancer Pathogenesis. Cancers (Basel) 11:N/A (2019). PubMed: 31357676
- Poser E et al. Aurora A promotes chromosome congression by activating the condensin-dependent pool of KIF4A. J Cell Biol 219:N/A (2019). PubMed: 31881080
- Abramo K et al. A chromosome folding intermediate at the condensin-to-cohesin transition during telophase. Nat Cell Biol 21:1393-1402 (2019). PubMed: 31685986
- Yokoyama Y et al. A novel role for the condensin II complex in cellular senescence. Cell Cycle 14:2160-70 (2015). PubMed: 26017022
- Liu W et al. PHF8 mediates histone H4 lysine 20 demethylation events involved in cell cycle progression. Nature 466:508-12 (2010). WB ; Human . PubMed: 20622854
- Centore RC et al. CRL4(Cdt2)-mediated destruction of the histone methyltransferase Set8 prevents premature chromatin compaction in S phase. Mol Cell 40:22-33 (2010). WB ; Human . PubMed: 20932472