概述

  • 产品名称Anti-H6PD抗体
    参阅全部 H6PD 一抗
  • 描述
    兔多克隆抗体to H6PD
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide, corresponding to a region within N terminal amino acids 179-228 (HFSAQQLATELGTFFQEEEMYRVDHYLGKQAVAQILPFRDQNRKALDGL W) of Human H6PD NP_0042760

  • 阳性对照
    • 721_B cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab84353 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 89 kDa (predicted molecular weight: 89 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P 1/100.

靶标

  • 功能Oxidizes glucose-6-phosphate and glucose, as well as other hexose-6-phosphates.
  • 组织特异性Present in most tissues examined, strongest in liver.
  • 疾病相关Defects in H6PD are a cause of cortisone reductase deficiency (CRD) [MIM:604931]. In CRD, activation of cortisone to cortisol does not occur, resulting in adrenocorticotropin-mediated androgen excess and a phenotype resembling polycystic ovary syndrome (PCOS).
  • 序列相似性In the N-terminal section; belongs to the glucose-6-phosphate dehydrogenase family.
    In the C-terminal section; belongs to the glucosamine/galactosamine-6-phosphate isomerase family. 6-phosphogluconolactonase subfamily.
  • 细胞定位Endoplasmic reticulum lumen. Microsomes, endoplasmic reticulum lumen.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 6 phosphogluconolactonase antibody
    • 6-phosphogluconolactonase antibody
    • 6PGL antibody
    • CORTRD1 antibody
    • G6PD, H form antibody
    • G6PDH antibody
    • G6PE_HUMAN antibody
    • GDH antibody
    • GDH/6PGL endoplasmic bifunctional protein antibody
    • Glucose 1 dehydrogenase antibody
    • Glucose 6 phosphate dehydrogenase, salivary antibody
    • Glucose dehydrogenase antibody
    • Glucose dehyrogenase antibody
    • Gpd1 antibody
    • H6pd antibody
    • Hexose 6 phosphate dehydrogenase (glucose 1 dehydrogenase) antibody
    • Hexose 6 phosphate dehydrogenase antibody
    • Hexose-6-phosphate dehydrogenase antibody
    see all

Anti-H6PD antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung tissue labelling H6PD with ab84353 at 1/100. A Cy3-conjugated donkey anti-rabbit IgG (1/200) was used as the secondary antibody. Positive staining shown in the cytoplasm of alveolar type I cells. Magnification: 20X. Exposure time: 0.5 - 2.0 seconds. Left - DAPI. Middle - H6PD. Right - Merge.
  • Anti-H6PD antibody (ab84353) at 1 µg/ml + 721_B cell lysate at 10 µg

    Secondary
    anti-Rabbit IgG HRP at 1/50000 dilution

    Predicted band size : 89 kDa
    Observed band size : 89 kDa
    Additional bands at : 42 kDa,65 kDa. We are unsure as to the identity of these extra bands.

Anti-H6PD antibody (ab84353)参考文献

ab84353 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Sheep Tissue lysate - whole (Liver)
Gel Running Conditions Reduced Denaturing (10% Tris Glycine Gels)
Loading amount 75 µg
Specification Liver
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. John Odhiambo

Verified customer

提交于 Sep 09 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"