概述

  • 产品名称Anti-H6PD抗体
    参阅全部 H6PD 一抗
  • 描述
    兔多克隆抗体to H6PD
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 600 - 700 of Human H6PD.

    (Peptide available as ab90254.)

  • 阳性对照
    • This antibody gave a positive signal in human liver tissue lysate.

性能

应用

Our Abpromise guarantee covers the use of ab72183 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 94 kDa (predicted molecular weight: 88 kDa).
ICC/IF Use a concentration of 5 µg/ml.

靶标

  • 功能Oxidizes glucose-6-phosphate and glucose, as well as other hexose-6-phosphates.
  • 组织特异性Present in most tissues examined, strongest in liver.
  • 疾病相关Defects in H6PD are a cause of cortisone reductase deficiency (CRD) [MIM:604931]. In CRD, activation of cortisone to cortisol does not occur, resulting in adrenocorticotropin-mediated androgen excess and a phenotype resembling polycystic ovary syndrome (PCOS).
  • 序列相似性In the N-terminal section; belongs to the glucose-6-phosphate dehydrogenase family.
    In the C-terminal section; belongs to the glucosamine/galactosamine-6-phosphate isomerase family. 6-phosphogluconolactonase subfamily.
  • 细胞定位Endoplasmic reticulum lumen. Microsomes, endoplasmic reticulum lumen.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 6 phosphogluconolactonase antibody
    • 6-phosphogluconolactonase antibody
    • 6PGL antibody
    • CORTRD1 antibody
    • G6PD, H form antibody
    • G6PDH antibody
    • G6PE_HUMAN antibody
    • GDH antibody
    • GDH/6PGL endoplasmic bifunctional protein antibody
    • Glucose 1 dehydrogenase antibody
    • Glucose 6 phosphate dehydrogenase, salivary antibody
    • Glucose dehydrogenase antibody
    • Glucose dehyrogenase antibody
    • Gpd1 antibody
    • H6pd antibody
    • Hexose 6 phosphate dehydrogenase (glucose 1 dehydrogenase) antibody
    • Hexose 6 phosphate dehydrogenase antibody
    • Hexose-6-phosphate dehydrogenase antibody
    see all

Anti-H6PD antibody 图像

  • Anti-H6PD antibody (ab72183) at 1 µg/ml + Human liver tissue lysate - total protein (ab29889) at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 88 kDa
    Observed band size : 94 kDa (why is the actual band size different from the predicted?)


    Exposure time : 2 minutes
  • ICC/IF image of ab72183 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab72183, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HepG2, and MCF-7 cells at 5µg/ml.

Anti-H6PD antibody (ab72183)参考文献

ab72183 has not yet been referenced specifically in any publications.

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