概述

  • 产品名称Anti-GW182抗体
    参阅全部 GW182 一抗
  • 描述
    兔多克隆抗体to GW182
  • 经测试应用适用于: ICC/IF, IHC-Pmore details
    不适用于: WB
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat
  • 免疫原

    A 16 amino acid peptide from near the C terminal residues of human GW182 protein (NM_020847; Q8NDV7).

  • 阳性对照
    • Brain lysates.

性能

  • 形式Lyophilised:Reconstitute with 200ul distilled sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA
  • Concentration information loading...
  • 纯度Protein A purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab84403 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration.
IHC-P Use a concentration of 1 µg/ml.
  • 应用说明Is unsuitable for WB.
  • 靶标

    • 功能Plays a role in RNA-mediated gene silencing by both micro-RNAs (miRNAs) and short interfering RNAs (siRNAs). Required for miRNA-dependent repression of translation and for siRNA-dependent endonucleolytic cleavage of complementary mRNAs by argonaute family proteins.
    • 组织特异性Ubiquitous.
    • 序列相似性Belongs to the GW182 family.
      Contains 1 RRM (RNA recognition motif) domain.
    • 细胞定位Cytoplasm > P-body. Mammalian P-bodies are also known as GW bodies.
    • Information by UniProt
    • 数据库链接
    • 别名
      • CAG repeat protein 26 antibody
      • CAGH26 antibody
      • DKFZp666E117 antibody
      • EDIE antibody
      • EMSY interactor protein antibody
      • FLJ22043 antibody
      • Glycine tryptophan protein of 182 kDa antibody
      • Glycine-tryptophan protein of 182 kDa antibody
      • GW1 antibody
      • GW182 antibody
      • GW182 autoantigen antibody
      • Hypothetical protein DKFZp566M143 antibody
      • KIAA1460 antibody
      • MGC75384 antibody
      • OTTHUMP00000122485 antibody
      • OTTHUMP00000195164 antibody
      • OTTHUMP00000195165 antibody
      • Protein GW1 antibody
      • TNR6A_HUMAN antibody
      • TNRC 6A antibody
      • TNRC6 antibody
      • Tnrc6a antibody
      • Trinucleotide repeat containing 6A antibody
      • Trinucleotide repeat containing gene 6A protein antibody
      • Trinucleotide repeat-containing gene 6A protein antibody
      see all

    Anti-GW182 antibody 图像

    • IHC image of ab84403 staining in Breast Cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab84403, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    Anti-GW182 antibody (ab84403)参考文献

    This product has been referenced in:
    • Aqil M  et al. The HIV-1 Nef protein binds argonaute-2 and functions as a viral suppressor of RNA interference. PLoS One 8:e74472 (2013). Read more (PubMed: 24023945) »
    • Chen S  et al. Ago-2-Mediated Slicer Activity Is Essential for Anti-Flaviviral Efficacy of RNAi. PLoS One 6:e27551 (2011). WB ; Human . Read more (PubMed: 22102908) »

    See all 2 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (HeLa)
    Specification HeLa
    Fixative Paraformaldehyde
    Permeabilization Yes - 0.5% triton x100
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
    Username

    Abcam user community

    Verified customer

    提交于 Oct 10 2012

    DISCOUNT CODE: [XXXXX] Expiration date: [07/03/2012] I am very pleased to hear you would like to accept our offer and test ab[87541] in [ICC/IF]. This code will give you 1 free [PRIMARY ANTIBODY] before the expiration date. To redeem this off...

    Read More

    Thank you for contacting us. We have 3 antibodies available in catalogue ab114857, ab87541 and ab84403. These antibodies are Rabbit polyclonal to GW182. Could you check the datasheets and select the product which is suitable for you. All of the...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Mouse Cell lysate - other (Mammary cell)
    Loading amount 50 µg
    Specification Mammary cell
    Treatment TGF beta for 3hrs
    Gel Running Conditions Reduced Denaturing (10)
    Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Username

    Abcam user community

    Verified customer

    提交于 Oct 11 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"