Anti-GW182抗体[4B6] - P/GW Body Marker (ab70522)

概述

  • 产品名称Anti-GW182抗体[4B6] - P/GW Body Marker
    参阅全部 GW182 一抗
  • 描述
    小鼠单克隆抗体[4B6] to GW182 - P/GW Body Marker
  • 经测试应用适用于: ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length protein minus the amino terminus TNR domain.

  • 阳性对照
    • HEp-2000 cells

性能

应用

Our Abpromise guarantee covers the use of ab70522 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/100.

靶标

  • 功能Plays a role in RNA-mediated gene silencing by both micro-RNAs (miRNAs) and short interfering RNAs (siRNAs). Required for miRNA-dependent repression of translation and for siRNA-dependent endonucleolytic cleavage of complementary mRNAs by argonaute family proteins.
  • 组织特异性Ubiquitous.
  • 序列相似性Belongs to the GW182 family.
    Contains 1 RRM (RNA recognition motif) domain.
  • 细胞定位Cytoplasm > P-body. Mammalian P-bodies are also known as GW bodies.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CAG repeat protein 26 antibody
    • CAGH26 antibody
    • DKFZp666E117 antibody
    • EDIE antibody
    • EMSY interactor protein antibody
    • FLJ22043 antibody
    • Glycine tryptophan protein of 182 kDa antibody
    • Glycine-tryptophan protein of 182 kDa antibody
    • GW1 antibody
    • GW182 antibody
    • GW182 autoantigen antibody
    • Hypothetical protein DKFZp566M143 antibody
    • KIAA1460 antibody
    • MGC75384 antibody
    • OTTHUMP00000122485 antibody
    • OTTHUMP00000195164 antibody
    • OTTHUMP00000195165 antibody
    • Protein GW1 antibody
    • TNR6A_HUMAN antibody
    • TNRC 6A antibody
    • TNRC6 antibody
    • Tnrc6a antibody
    • Trinucleotide repeat containing 6A antibody
    • Trinucleotide repeat containing gene 6A protein antibody
    • Trinucleotide repeat-containing gene 6A protein antibody
    see all

Anti-GW182 antibody [4B6] - P/GW Body Marker 图像

  • ab70522 staining GW182 in pre-fixed HEp-2000 cells. ab70522 was used at 1/100 dilution and incubated overnight at 4°C. An Alexa Fluor® 488-conjugated anti-mouse IgG was used as the secondary antibody.

  • ab70522 (1/200) staining GW182 in assynchronous HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilised with Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (red). Please refer to abreview for further experimental details.

    See Abreview

Anti-GW182 antibody [4B6] - P/GW Body Marker (ab70522)参考文献

This product has been referenced in:
  • Li S  et al. TRIM65 regulates microRNA activity by ubiquitination of TNRC6. Proc Natl Acad Sci U S A 111:6970-5 (2014). Read more (PubMed: 24778252) »
  • Moser JJ & Fritzler MJ Cytoplasmic ribonucleoprotein (RNP) bodies and their relationship to GW/P bodies. Int J Biochem Cell Biol 42:828-43 (2010). Read more (PubMed: 19944184) »

See all 7 Publications for this product

Product Wall


Unfortunately, I have been informed that the ab70522 Anti-GW182 antibody [4B6] - P/GW Body Marker has not been tested in Xenopus laevis. This was an error and our datasheet has now been updated so that Xenopus laevis is no longer listed as a tes...

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DISCOUNT CODE:***
Expiration date: January 24, 2013

This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview forAb87541 in ICC/IFand include this code in the “Addit...

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DISCOUNT CODE: [XXXXX] Expiration date: [07/03/2012] I am very pleased to hear you would like to accept our offer and test ab[87541] in [ICC/IF]. This code will give you 1 free [PRIMARY ANTIBODY] before the expiration date. To redeem this off...

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Thank you for contacting us. We have 3 antibodies available in catalogue ab114857, ab87541 and ab84403. These antibodies are Rabbit polyclonal to GW182. Could you check the datasheets and select the product which is suitable for you. All of the...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% TritonX100 in PBS
Username

Dr. Kirk McManus

Verified customer

提交于 May 22 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"