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Our Abpromise guarantee covers the use of ab70522 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab70522 staining GW182 in pre-fixed HEp-2000 cells. ab70522 was used at 1/100 dilution and incubated overnight at 4°C. An Alexa Fluor® 488-conjugated anti-mouse IgG was used as the secondary antibody.