Anti-GUCY1B3抗体(ab24824)
Key features and details
- Rabbit polyclonal to GUCY1B3
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-GUCY1B3抗体
参阅全部 GUCY1B3 一抗 -
描述
兔多克隆抗体to GUCY1B3 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Dog, Pig -
免疫原
Synthetic peptide corresponding to GUCY1B3.
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阳性对照
- Brain
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常规说明
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab24824于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF |
Use a concentration of 5 µg/ml.
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IHC-P |
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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说明 |
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ICC/IF
Use a concentration of 5 µg/ml. |
IHC-P
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
靶标
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序列相似性
Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.
Contains 1 guanylate cyclase domain. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 2983 Human
- Entrez Gene: 54195 Mouse
- Entrez Gene: 25202 Rat
- Omim: 139397 Human
- SwissProt: Q02153 Human
- SwissProt: O54865 Mouse
- SwissProt: P20595 Rat
- Unigene: 77890 Human
see all -
别名
- GC-S-beta-1 antibody
- GC-SB3 antibody
- GCbeta1 antibody
see all
图片
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Human cerebellum stained with Anti GUCY1B3 ab24824 at 1/100 dilution for 30 min at RT.
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ICC/IF image of ab24824 stained SKNSH cells. The cells were Both and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab24824, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (8)
ab24824 被引用在 8 文献中.
- de Winde CM et al. Fibroblastic reticular cell response to dendritic cells requires coordinated activity of podoplanin, CD44 and CD9. J Cell Sci 134:N/A (2021). PubMed: 34184727
- de Winde CM et al. Podoplanin drives dedifferentiation and amoeboid invasion of melanoma. iScience 24:102976 (2021). PubMed: 34485858
- Lukácová N et al. Is Innervation of the Neuromuscular Junction at the Diaphragm Modulated by sGC/cGMP Signaling? Front Physiol 11:700 (2020). PubMed: 32655417
- Waddingham MT et al. Diastolic dysfunction is initiated by cardiomyocyte impairment ahead of endothelial dysfunction due to increased oxidative stress and inflammation in an experimental prediabetes model. J Mol Cell Cardiol 137:119-131 (2019). PubMed: 31669609
- Maruyama K et al. Age-related changes to vascular protease-activated receptor 2 in metabolic syndrome: a relationship between oxidative stress, receptor expression, and endothelium-dependent vasodilation. Can J Physiol Pharmacol 95:356-364 (2017). PubMed: 28103056
- Tsihlis ND et al. Nitric oxide is less effective at inhibiting neointimal hyperplasia in spontaneously hypertensive rats. Nitric Oxide 35:165-74 (2013). IHC-P ; Rat . PubMed: 24149190
- van Heerebeek L et al. Low myocardial protein kinase g activity in heart failure with preserved ejection fraction. Circulation 126:830-9 (2012). IHC-P ; Human . PubMed: 22806632
- Succu S et al. Oxytocin induces penile erection when injected into the ventral tegmental area of male rats: role of nitric oxide and cyclic GMP. Eur J Neurosci 28:813-21 (2008). IHC-FoFr ; Rat . PubMed: 18671741