The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC (PFA fixed)
IHC-Fr: Use at an assay dependent dilution.
Immunohistochemistry (PFA fixed): Use at an assay dependent dilution.
WB: 1/500. Detects a band of approximately 98 kDa (predicted molecular weight: 98 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Has guanylyl cyclase on binding to the beta-1 subunit. Isoform 2 acts as a negative regulator of guanylyl cyclase activity as it forms non-functional heterodimers with the beta subunits.
Isoform 1 is expressed in fetal brain, liver, colon, endothelium and testis. Isoform 2 is expressed only in liver, colon and endothelium.
Belongs to the adenylyl cyclase class-4/guanylyl cyclase family. Contains 1 guanylate cyclase domain.