Anti-Guanylyl Cyclase alpha 2抗体(ab42108)

概述

  • 产品名称
    Anti-Guanylyl Cyclase alpha 2抗体
  • 描述
    兔多克隆抗体to Guanylyl Cyclase alpha 2
  • 经测试应用
    适用于: IHC-Fr, WB, IHC (PFA fixed)more details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide:

    GICYFLEVR TGPKPPKP

    , corresponding to amino acids 692-708 of Human Guanylyl Cyclase alpha 2

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Antibody stabilization buffer, with preservatives, consisting of Hepes, NaCl, KCl, glycerol, BSA and chaotropic agents.
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab42108 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr
WB
IHC (PFA fixed)
  • 应用说明
    IHC-Fr: Use at an assay dependent dilution.
    Immunohistochemistry (PFA fixed): Use at an assay dependent dilution.
    WB: 1/500. Detects a band of approximately 98 kDa (predicted molecular weight: 98 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能
      Has guanylyl cyclase on binding to the beta-1 subunit.
      Isoform 2 acts as a negative regulator of guanylyl cyclase activity as it forms non-functional heterodimers with the beta subunits.
    • 组织特异性
      Isoform 1 is expressed in fetal brain, liver, colon, endothelium and testis. Isoform 2 is expressed only in liver, colon and endothelium.
    • 序列相似性
      Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.
      Contains 1 guanylate cyclase domain.
    • 细胞定位
      Cytoplasm.
    • Information by UniProt
    • 数据库链接
    • 别名
      • GC SA2 antibody
      • GCS alpha 2 antibody
      • GCS-alpha-2 antibody
      • GCYA2_HUMAN antibody
      • Guanylate cyclase soluble subunit alpha-2 antibody
      • GUC1A2 antibody
      • GUCSA2 antibody
      • GUCY1A2 antibody
      see all

    图片

    • Anti-Guanylyl Cyclase alpha 2 antibody (ab42108) at 1/750 dilution + PC-soluble guanylate cyclase alfa 2

      Predicted band size : 98 kDa
      Observed band size : 98 kDa
    • Immunodetection of guanylyl cyclase alpha 2 in mouse brain tissue section using ab42108 (1/100). Please refer to abreview for further experimental details.

      See Abreview

    文献

    This product has been referenced in:
    • Thoonen R  et al. Cardiovascular and pharmacological implications of haem-deficient NO-unresponsive soluble guanylate cyclase knock-in mice. Nat Commun 6:8482 (2015). Read more (PubMed: 26442659) »
    • Buys ES  et al. sGC(alpha)1(beta)1 attenuates cardiac dysfunction and mortality in murine inflammatory shock models. Am J Physiol Heart Circ Physiol 297:H654-63 (2009). WB ; Mouse . Read more (PubMed: 19502556) »

    See all 4 Publications for this product

    客户评价及客户问答

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (brain)
    Specification
    brain
    Fixative
    Paraformaldehyde
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 10% · Temperature: 20°C
    Username

    Ute Neubacher

    Verified customer

    提交于 Feb 17 2009

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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