Anti-Guanylyl Cyclase alpha 1抗体(ab85445)

概述

  • 产品名称Anti-Guanylyl Cyclase alpha 1抗体
    参阅全部 Guanylyl Cyclase alpha 1 一抗
  • 描述
    兔多克隆抗体to Guanylyl Cyclase alpha 1
  • 经测试应用适用于: ICC/IF, IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Rat
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Rat Guanylyl Cyclase alpha 1.

    (Peptide available as ab97987.)

  • 阳性对照
    • This antibody gave a positive signal in the following tissue lysates: Rat kidney; Rat lung; Mouse lung; Rat heart. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HeLa.

性能

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应用

Our Abpromise guarantee covers the use of ab85445 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 77 kDa (predicted molecular weight: 77 kDa).

靶标

  • 序列相似性Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.
    Contains 1 guanylate cyclase domain.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • GC S alpha 1 antibody
    • GC SA3 antibody
    • GCS alpha 1 antibody
    • GCS alpha 3 antibody
    • GCS-alpha-1 antibody
    • GCS-alpha-3 antibody
    • GCYA3_HUMAN antibody
    • Guanylate cyclase 1 soluble alpha 3 antibody
    • Guanylate cyclase soluble subunit alpha 1 antibody
    • Guanylate cyclase soluble subunit alpha 3 antibody
    • Guanylate cyclase soluble subunit alpha-3 antibody
    • Guc1a1 antibody
    • GUC1A3 antibody
    • GUCA3 antibody
    • GUCSA3 antibody
    • Gucy1a1 antibody
    • Gucy1a3 antibody
    • Soluble guanylate cyclase large subunit antibody
    see all

Anti-Guanylyl Cyclase alpha 1 antibody 图像

  • All lanes : Anti-Guanylyl Cyclase alpha 1 antibody (ab85445) at 1 µg/ml

    Lane 1 : Kidney (Rat) Tissue Lysate
    Lane 2 : Lung (Rat) Tissue Lysate
    Lane 3 : Mouse lung normal tissue lysate - total protein (ab29297)
    Lane 4 : Heart (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 77 kDa
    Observed band size : 77 kDa
    Additional bands at : 48 kDa,58 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes
  • IHC image of Guanylyl Cyclase alpha 1 staining in human Normal Kidney FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab85445, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX
  • ICC/IF image of ab85445 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab85445 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Guanylyl Cyclase alpha 1 antibody (ab85445)参考文献

ab85445 has not yet been referenced specifically in any publications.

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