Anti-Guanylate Cyclase抗体[GC25] (ab20042)

概述

  • 产品名称
    Anti-Guanylate Cyclase抗体[GC25]
    参阅全部 Guanylate Cyclase 一抗
  • 描述
    小鼠单克隆抗体[GC25] to Guanylate Cyclase
  • 特异性
    Ab20042 recognizes the beta subunit of Human Soluble Guanylate Cyclase.
  • 经测试应用
    适用于: Flow Cyt, ELISA, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length protein: Recombinant human soluble guanylate cyclase alpha1 beta1 heterodimer.

  • 常规说明

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 纯度
    Protein A purified
  • 纯化说明
    Ab20042 is purified from ascites.
  • 克隆
    单克隆
  • 克隆编号
    GC25
  • 骨髓瘤
    Sp2/0
  • 同种型
    IgG2a
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab20042 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 0.5µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
ELISA Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 70 kDa.

靶标

  • 相关性
    Guanylate Cyclase is an heterodimer of an alpha and a beta chain. Its enzymatic activity transforms GTP to GMP releasing a diphosphate. It binds 1 or 2 hemes per heterodimer. It is activated by nitric oxide in the presence of magnesium or manganese ions. There are two types of guanylate cyclase: soluble form and membrane-associated receptor form.
  • 细胞定位
    Cytoplasmic
  • 数据库链接
  • 别名
    • GC SB2 antibody
    • GCS beta 2 antibody
    • Guanylate cyclase 1 soluble beta 2 antibody
    • GUCY1B2 antibody

Anti-Guanylate Cyclase antibody [GC25] 图像

  • Overlay histogram showing HeLa cells stained with ab20042 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab20042, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-Guanylate Cyclase antibody [GC25] (ab20042)参考文献

This product has been referenced in:
  • Bahls M  et al. Mother's exercise during pregnancy programmes vasomotor function in adult offspring. Exp Physiol 99:205-19 (2014). Human . Read more (PubMed: 24163423) »

See 1 Publication for this product

Product Wall

Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Sample
Rat Tissue sections (retina)
Specification
retina
Permeabilization
Yes - Triton X-100 0.3%
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Jun 03 2014

The originator of this antibody used a purified human soluble guanylate cyclase as a positive control antigen in Western blot testing. Unfortunately, this antigen is not available for sale. If you have any additional questions, please contact us ag...

Read More

Thank you for your enquiry. I would suggest a beta actin loading control (such as ab8227) or GAPDH (such as ab9485). If you have any additional questions, please contact us again.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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