Anti-Guanylate Cyclase抗体[GC25] (ab20042)


  • 产品名称Anti-Guanylate Cyclase抗体[GC25]
    参阅全部 Guanylate Cyclase 一抗
  • 描述
    小鼠单克隆抗体[GC25] to Guanylate Cyclase
  • 特异性Ab20042 recognizes the beta subunit of Human Soluble Guanylate Cyclase.
  • 经测试应用适用于: Flow Cyt, ELISA, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length protein: Recombinant human soluble guanylate cyclase alpha1 beta1 heterodimer.


  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 纯度Protein A purified
  • 纯化说明Ab20042 is purified from ascites.
  • 克隆单克隆
  • 克隆编号GC25
  • 骨髓瘤Sp2/0
  • 同种型IgG2a
  • 研究领域


Our Abpromise guarantee covers the use of ab20042 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 0.5µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
ELISA Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 70 kDa.


  • 相关性Guanylate Cyclase is an heterodimer of an alpha and a beta chain. Its enzymatic activity transforms GTP to GMP releasing a diphosphate. It binds 1 or 2 hemes per heterodimer. It is activated by nitric oxide in the presence of magnesium or manganese ions. There are two types of guanylate cyclase: soluble form and membrane-associated receptor form.
  • 细胞定位Cytoplasmic
  • 数据库链接
  • 别名
    • GC SB2 antibody
    • GCS beta 2 antibody
    • Guanylate cyclase 1 soluble beta 2 antibody
    • GUCY1B2 antibody

Anti-Guanylate Cyclase antibody [GC25] 图像

  • Overlay histogram showing HeLa cells stained with ab20042 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab20042, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-Guanylate Cyclase antibody [GC25] (ab20042)参考文献

This product has been referenced in:
  • Bahls M  et al. Mother's exercise during pregnancy programmes vasomotor function in adult offspring. Exp Physiol 99:205-19 (2014). Human . Read more (PubMed: 24163423) »

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Sample Rat Tissue sections (retina)
Specification retina
Permeabilization Yes - Triton X-100 0.3%
Fixative Paraformaldehyde

Abcam user community

Verified customer

提交于 Jun 03 2014

The originator of this antibody used a purified human soluble guanylate cyclase as a positive control antigen in Western blot testing. Unfortunately, this antigen is not available for sale. If you have any additional questions, please contact us ag...

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Thank you for your enquiry. I would suggest a beta actin loading control (such as ab8227) or GAPDH (such as ab9485). If you have any additional questions, please contact us again.