KLH conjugated synthetic peptide corresponding to a region within C terminal amino acids 691-720 of Human GTSE1.
Hela cell lysate; Human brain tissue
Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
Preservative: 0.09% Sodium Azide
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Immunogen affinity purified
ab103232 is purified through a protein A column, followed by peptide affinity purification.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/500. Predicted molecular weight: 77 kDa.
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
GTSE1 is only expressed in the S and G2 phases of the cell cycle, where it co-localizes with cytoplasmic tubulin and microtubules. In response to DNA damage, GTSE1 accumulates in the nucleus and binds the tumor suppressor protein p53, shuttling it out of the nucleus and repressing its ability to induce apoptosis. Overexpression of GTSE1 delays G2/M phase progression.
Cytoplasm. Note: Associated with microtubules
B99 homolog antibody
G 2 and S phase expressed 1 antibody
Anti-GTSE1 antibody 图像
Western blot - GTSE1 antibody (ab103232)
Anti-GTSE1 antibody (ab103232) at 1/100 dilution + Hela cell lysate at 35 µg
Predicted band size : 77 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GTSE1 antibody (ab103232)
ab103232, at 1/50 dilution, staining GTSE1 in formalin fixed, paraffin embedded Human brain tissue by Immunohistochemistry, using a peroxidase conjugated secondary antibody and DAB staining.
Anti-GTSE1 antibody (ab103232)参考文献
has not yet been referenced specifically in any publications.
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