Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
A region within synthetic peptide: QGLGNAFLSH ISACDGIFHL TRAFEDDDIT HVEGSVDPIR DIEIIHEELQ, corresponding to amino acids 104-153 of Human GTPBP9
Jurkat cell lysate, Human intestine
This antibody gave a positive result in IF in the following Formaldehyde/Methanol fixed cell line: HCT116
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Constituents: 2% Sucrose, PBS
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.25 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use a concentration of 4 - 8 µg/ml.
Use a concentration of 5 µg/ml.
Hydrolyzes ATP, and can also hydrolyze GTP with lower efficiency. Has lower affinity for GTP.
Belongs to the GTP1/OBG family.
Contains 1 G (guanine nucleotide-binding) domain.
Information by UniProt
DNA damage-regulated overexpressed in cancer 45 protein antibody
Western blot - GTPBP9 antibody (ab51077)
Lane 1 : Marker Lane 2 : Anti-GTPBP9 antibody (ab51077) at 0.25 µg/ml Lane 1 : As above Lane 2 : Jurkat cell lysate at 10 µg/ml with skim milk/ PBS at 5 % Secondary Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution Predicted band size : 45 kDa Observed band size : 45 kDa
Immunohistochemistry (Paraffin-embedded sections) - GTPBP9 antibody (ab51077)
ab51077 at 4.0 µg/ml staining epithelial cells of the intestinal villus from human intestine; paraffin embedded; magnification 400x.
Immunocytochemistry/ Immunofluorescence - Anti-GTPBP9 antibody (ab51077)
ab51077 stained HCT116 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab51077 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (
) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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