Anti-GTPase HRAS抗体[Y132] (ab32417)


  • 产品名称Anti-GTPase HRAS抗体[Y132]
    参阅全部 GTPase HRAS 一抗
  • 描述
    兔单克隆抗体[Y132] to GTPase HRAS
  • 特异性Reactivity with other RAS members has not been tested.
  • 经测试应用适用于: WB, IPmore details
    不适用于: IHC
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Chicken
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human GTPase HRAS aa 150 to the C-terminus (C terminal).

  • 阳性对照
    • MCF7 and PC12 cell lysates and MCF7 cells.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.


    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.



Our Abpromise guarantee covers the use of ab32417 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 21 kDa.
IP 1/50 - 1/60.
  • 应用说明Is unsuitable for IHC.
  • 靶标

    • 功能Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.
    • 疾病相关Defects in HRAS are the cause of faciocutaneoskeletal syndrome (FCSS) [MIM:218040]. A rare condition characterized by prenatally increased growth, postnatal growth deficiency, mental retardation, distinctive facial appearance, cardiovascular abnormalities (typically pulmonic stenosis, hypertrophic cardiomyopathy and/or atrial tachycardia), tumor predisposition, skin and musculoskeletal abnormalities.
      Defects in HRAS are the cause of congenital myopathy with excess of muscle spindles (CMEMS) [MIM:218040]. CMEMS is a variant of Costello syndrome.
      Defects in HRAS may be a cause of susceptibility to Hurthle cell thyroid carcinoma (HCTC) [MIM:607464]. Hurthle cell thyroid carcinoma accounts for approximately 3% of all thyroid cancers. Although they are classified as variants of follicular neoplasms, they are more often multifocal and somewhat more aggressive and are less likely to take up iodine than are other follicular neoplasms.
      Note=Mutations which change positions 12, 13 or 61 activate the potential of HRAS to transform cultured cells and are implicated in a variety of human tumors.
      Defects in HRAS are a cause of susceptibility to bladder cancer (BLC) [MIM:109800]. A malignancy originating in tissues of the urinary bladder. It often presents with multiple tumors appearing at different times and at different sites in the bladder. Most bladder cancers are transitional cell carcinomas. They begin in cells that normally make up the inner lining of the bladder. Other types of bladder cancer include squamous cell carcinoma (cancer that begins in thin, flat cells) and adenocarcinoma (cancer that begins in cells that make and release mucus and other fluids). Bladder cancer is a complex disorder with both genetic and environmental influences.
      Note=Defects in HRAS are the cause of oral squamous cell carcinoma (OSCC).
    • 序列相似性Belongs to the small GTPase superfamily. Ras family.
    • 翻译后修饰Palmitoylated by the ZDHHC9-GOLGA7 complex. A continuous cycle of de- and re-palmitoylation regulates rapid exchange between plasma membrane and Golgi.
      S-nitrosylated; critical for redox regulation. Important for stimulating guanine nucleotide exchange. No structural perturbation on nitrosylation.
    • 细胞定位Cell membrane. Golgi apparatus membrane. The active GTP-bound form is localized most strongly to membranes than the inactive GDP-bound form (By similarity). Shuttles between the plasma membrane and the Golgi apparatus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • C BAS/HAS antibody
      • c H ras antibody
      • C HA RAS1 antibody
      • c has/bas p21 protein antibody
      • c ras Ki 2 activated oncogene antibody
      • c-H-ras antibody
      • CTLO antibody
      • GTP and GDP binding peptide B antibody
      • GTPase HRas, N-terminally processed antibody
      • H Ras 1 antibody
      • H RASIDX antibody
      • H-Ras-1 antibody
      • Ha Ras antibody
      • Ha Ras1 proto oncoprotein antibody
      • Ha-Ras antibody
      • HAMSV antibody
      • Harvey rat sarcoma viral oncogene homolog antibody
      • Harvey rat sarcoma viral oncoprotein antibody
      • HRAS antibody
      • HRAS1 antibody
      • K ras antibody
      • N ras antibody
      • p19 H RasIDX protein antibody
      • p21ras antibody
      • Ras family small GTP binding protein H Ras antibody
      • RASH_HUMAN antibody
      • RASH1 antibody
      • Transformation gene oncogene HAMSV antibody
      • Transforming protein p21 antibody
      • v Ha ras Harvey rat sarcoma viral oncogene homolog antibody
      • VH Ras antibody
      • vHa RAS antibody
      see all

    Anti-GTPase HRAS antibody [Y132] 图像

    • All lanes : Anti-GTPase HRAS antibody [Y132] (ab32417) at 1/2500 dilution (purified)

      Lane 1 : mouse brain lysate
      Lane 2 : rat brain lysate

      Lysates/proteins at 10 µg per lane.

      HRP goat anti-rabbit IgG (H+L) at 1/20000 dilution

      Observed band size : 21 kDa (why is the actual band size different from the predicted?)

      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • All lanes : Anti-GTPase HRAS antibody [Y132] (ab32417) at 1/1000 dilution (purified)

      Lane 1 : MCF7 cell lysate
      Lane 2 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      HRP goat anti-rabbit IgG (H+L) at 1/20000 dilution

      Observed band size : 21 kDa (why is the actual band size different from the predicted?)

      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • ab32417 (purified) at 1/60 immunoprecipitating GTPase in 10 μg mouse brain whole cell lysate (Lanes 1 and 2, observed at 21 kDa). Lane 3 - PBS. For western blotting, HRP Veriblot for IP (ab131366) was used as the secondary antibody (1/10 000). Blocking buffer and concentration: 5% NFDM/TBST Dilution buffer and concentration: 5% NFDM/TBST
    • All lanes : Anti-GTPase HRAS antibody [Y132] (ab32417) at 1/500 dilution (unpurified)

      Lane 1 : MCF7 cell lysate
      Lane 2 : PC12 cell lysate

      Observed band size : 21 kDa (why is the actual band size different from the predicted?)

    Anti-GTPase HRAS antibody [Y132] (ab32417)参考文献

    This product has been referenced in:
    • Aguilera-Aguirre L  et al. Innate inflammation induced by the 8-oxoguanine DNA glycosylase-1-KRAS-NF-?B pathway. J Immunol 193:4643-53 (2014). WB . Read more (PubMed: 25267977) »
    • Haeussler DJ  et al. Endomembrane H-Ras controls vascular endothelial growth factor-induced nitric-oxide synthase-mediated endothelial cell migration. J Biol Chem 288:15380-9 (2013). WB ; Human . Read more (PubMed: 23548900) »

    See all 6 Publications for this product

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Mouse Cell lysate - whole cell (Colon cancer cells)
    Loading amount 100 µg
    Specification Colon cancer cells
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

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    提交于 Oct 17 2011