Anti-GTF3C5抗体(ab74305)

概述

  • 产品名称Anti-GTF3C5抗体
    参阅全部 GTF3C5 一抗
  • 描述
    兔多克隆抗体to GTF3C5
  • 经测试应用适用于: WB, IP, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus (Ornithorhynchus anatinus)
  • 免疫原

    Synthetic peptide from a region between residue 469 and 519 of human GTF3C5 (using the numbering given in entry NP_036219.1)

  • 阳性对照
    • Lysate from HeLa, 293T or NIH3T3 cells

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab74305 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 60 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

Anti-GTF3C5 antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma (left) and mouse teratoma (right) tissues labelling GTF3C5 with ab74305 at 1/500 (0.4µg/ml). Detection: DAB.
  • All lanes : Anti-GTF3C5 antibody (ab74305) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg


    Predicted band size : 60 kDa
    Observed band size : 60 kDa
    Additional bands at : 90 kDa. We are unsure as to the identity of these extra bands.
  • 20% of immunoprecipitate obtained from 1mg HeLa whole cell lysate using 3µg ab74305 or a control IgG was loaded in lane 1 and 2 respectively. GTF3C5 was then detected by WB, using ab74305 at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 1 second.

Anti-GTF3C5 antibody (ab74305)参考文献

ab74305 has not yet been referenced specifically in any publications.

Product Wall

Application ChIP
Detection step Real-time PCR
Sample Mouse Cell lysate - whole cell (NIH 3T3)
Specification NIH 3T3
Negative control RNA Pol II genes
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: Formaldehyde
Positive control RNA Pol III genes
Username

Ms. Jennifer Blancas

Verified customer

提交于 Jul 14 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"