概述

  • 产品名称Anti-GTF3C4抗体
    参阅全部 GTF3C4 一抗
  • 描述
    兔多克隆抗体to GTF3C4
  • 经测试应用适用于: WB, IP, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region between residue 1 and 50 of human GTF3C4 (NP_036336.1)

  • 阳性对照
    • HeLa whole cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab74229 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 92 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Essential for RNA polymerase III to make a number of small nuclear and cytoplasmic RNAs, including 5S RNA, tRNA, and adenovirus-associated (VA) RNA of both cellular and viral origin. Has histone acetyltransferase activity (HAT) with unique specificity for free and nucleosomal H3. May cooperate with GTF3C5 in facilitating the recruitment of TFIIIB and RNA polymerase through direct interactions with BRF1, POLR3C and POLR3F. May be localized close to the A box.
  • 序列相似性Belongs to the TFIIIC subunit 4 family.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • EC 2.3.1.48 antibody
    • FLJ21002 antibody
    • General transcription factor 3C 4 antibody
    • General transcription factor 3C polypeptide 4 antibody
    • General transcription factor IIIC, polypeptide 4, 90kDa antibody
    • GTF3C4 antibody
    • KAT12 antibody
    • MGC138450 antibody
    • OTTHUMP00000022434 antibody
    • TF3C delta antibody
    • TF3C-delta antibody
    • TF3C4_HUMAN antibody
    • TFIII90 antibody
    • TFIIIC 90 kDa subunit antibody
    • TFiiiC2 90 antibody
    • TFIIIC290 antibody
    • TFIIIC90 antibody
    • TFIIICdelta antibody
    • Transcription factor IIIC 90 kDa subunit antibody
    • Transcription factor IIIC subunit delta antibody
    see all

Anti-GTF3C4 antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human thyroid carcinoma tissue labelling GTF3C4 with ab74229 at 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-GTF3C4 antibody (ab74229) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg

    Developed using the ECL technique

    Predicted band size : 92 kDa
    Observed band size : 95 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 185 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 10 seconds
  • ab74229, at 1 µg/ml (used for IP at 3 µg/mg lysate)
    Lane 1: HeLa whole cell lysate at 1 mg for IP (20% of IP loaded).
    Lane 2: control IgG.
    Detection: Chemiluminescence with an exposure time of 3 seconds.

Anti-GTF3C4 antibody (ab74229)参考文献

ab74229 has not yet been referenced specifically in any publications.

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