概述

  • 产品名称Anti-gtf3c3抗体
    参阅全部 gtf3c3 一抗
  • 描述
    兔多克隆抗体to gtf3c3
  • 经测试应用适用于: WB, IP, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
  • 免疫原

    Synthetic peptide corresponding to a region between residue 475 and 525 of human gtf3c3 (NP_036218.1)

  • 阳性对照
    • HeLa whole cell lysate. Mouse NIH3T3 whole Cell Lysate.

性能

应用

Our Abpromise guarantee covers the use of ab74230 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 101 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Involved in RNA polymerase III-mediated transcription. Integral, tightly associated component of the DNA-binding TFIIIC2 subcomplex that directly binds tRNA and virus-associated RNA promoters.
  • 序列相似性Contains 11 TPR repeats.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • General transcription factor 3C polypeptide 3 antibody
    • general transcription factor IIIC, polypeptide 3 (102kD) antibody
    • general transcription factor IIIC, polypeptide 3, 102kD antibody
    • GTF3C3 antibody
    • TF3C-gamma antibody
    • TF3C3_HUMAN antibody
    • TFIIIC 102 kDa subunit antibody
    • TFIIIC gamma antibody
    • TFIIIC102 antibody
    • TFIIIC2 102 antibody
    • TFIIICgamma antibody
    • Transcription factor IIIC 102 kDa subunit antibody
    • transcription factor IIIC gamma subunit antibody
    • Transcription factor IIIC subunit gamma antibody
    • transcription factor IIIC, 102 kD antibody
    see all

Anti-gtf3c3 antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling gtf3c3 with ab74230 at 1/500 (0.4µg/ml). Detection: DAB.
  • All lanes : Anti-gtf3c3 antibody (ab74230) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T cell at 50 µg
    Lane 5 : mouse NIH3T3 cell at 50 µg


    Predicted band size : 101 kDa
    Observed band size : 101 kDa


    Exposure time : 3 minutes
  • Lane 1: HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) with ab74230 used for IP at 3 µg/mg lysate.
    Lane 2: control IgG.
    Subsequent Western blot detection peformed using ab74230 at 1 µg/ml.
    Detection: Chemiluminescence with an exposure time of 3 minutes.

Anti-gtf3c3 antibody (ab74230)参考文献

ab74230 has not yet been referenced specifically in any publications.

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