Anti-GTF3C2抗体(ab89113)

概述

  • 产品名称
  • 描述
    小鼠多克隆抗体to GTF3C2
  • 经测试应用
    适用于: ChIP, WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length GTF3C2 protein (NP_001030598.1).

  • 阳性对照
    • HeLa cell lysate, GTF3C2 transfected 293T cell line lysate and HeLa cells.

性能

应用

Our Abpromise guarantee covers the use of ab89113 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ChIP 1/100.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 101 kDa.
ICC/IF Use a concentration of 10 µg/ml.

靶标

  • 功能
    Required for RNA polymerase III-mediated transcription. Component of TFIIIC that initiates transcription complex assembly on tRNA and is required for transcription of 5S rRNA and other stable nuclear and cytoplasmic RNAs. May play a direct role in stabilizing interactions of TFIIIC2 with TFIIIC1.
  • 序列相似性
    Contains 4 WD repeats.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • General transcription factor 3C polypeptide 2 antibody
    • General transcription factor IIIC polypeptide 2 beta 110kDa antibody
    • Gtf3c2 antibody
    • KIAA0011 antibody
    • TF3C beta antibody
    • TF3C-beta antibody
    • TF3C2_HUMAN antibody
    • TFIIIC 110 kDa subunit antibody
    • TFIIIC BETA antibody
    • TFIIIC110 antibody
    • Transcription factor IIIC 110 kDa subunit antibody
    • Transcription factor IIIC subunit beta antibody
    see all

图片

  • ChIP analysis using ab89113 binding GTF3C2 in iPS derived from Human neuronal. Cells were cross-linked for 10 minutes with  formaldehyde. Samples were incubated with primary antibody(1/100) for 16 hours at 4°C. Protein binding was detected using real-time PCR.
    Positive control: 7SL RNA gene

    Negative Control: GAPDH coding region.

     

    See Abreview

  • Anti-GTF3C2 antibody (ab89113) at 1 µg/ml + HeLa cell lysate at 50 µg

    Predicted band size : 101 kDa
    Observed band size : 101 kDa
  • All lanes : Anti-GTF3C2 antibody (ab89113) at 1 µg/ml

    Lane 1 : GTF3C2 transfected 293T cell line lysate
    Lane 2 : Non-transfected lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 101 kDa
    Observed band size : 125 kDa (why is the actual band size different from the predicted?)
  • ab89113, at 10 µg/ml staining GTF3C2 in HeLa cells by Immunofluorescence.

文献

ab89113 has not yet been referenced specifically in any publications.

客户评价及客户问答

Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing (Gradientgel 4-12)
Sample
Mouse Cell lysate - whole cell (Hela,HEK293, fibroblasts, ES cells)
Specification
Hela,HEK293, fibroblasts, ES cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Oct 14 2013

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
ChIP
Sample
Human Cell lysate - whole cell (iPS derived neuronal cells)
Specification
iPS derived neuronal cells
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: Formaldehyde
Detection step
Real-time PCR
Positive control
7SL RNA gene
Negative control
GAPDH coding region
Username

Abcam user community

Verified customer

提交于 Apr 11 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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