Anti-TFIIIC110抗体(ab89113)
Key features and details
- Mouse polyclonal to TFIIIC110
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-TFIIIC110抗体 -
描述
小鼠多克隆抗体to TFIIIC110 -
宿主
Mouse -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Recombinant full length protein within Human TFIIIC110. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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ChIP Related Products
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab89113于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (1) |
Use a concentration of 1 µg/ml. Predicted molecular weight: 101 kDa.
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ICC/IF |
Use a concentration of 10 µg/ml.
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说明 |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 101 kDa. |
ICC/IF
Use a concentration of 10 µg/ml. |
靶标
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功能
Required for RNA polymerase III-mediated transcription. Component of TFIIIC that initiates transcription complex assembly on tRNA and is required for transcription of 5S rRNA and other stable nuclear and cytoplasmic RNAs. May play a direct role in stabilizing interactions of TFIIIC2 with TFIIIC1. -
序列相似性
Contains 4 WD repeats. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 2976 Human
- Omim: 604883 Human
- SwissProt: Q8WUA4 Human
- Unigene: 75782 Human
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别名
- General transcription factor 3C polypeptide 2 antibody
- General transcription factor IIIC polypeptide 2 beta 110kDa antibody
- Gtf3c2 antibody
see all
图片
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Anti-TFIIIC110 antibody (ab89113) at 1 µg/ml + HeLa cell lysate at 50 µg
Predicted band size: 101 kDa
Observed band size: 101 kDa -
All lanes : Anti-TFIIIC110 antibody (ab89113) at 1 µg/ml
Lane 1 : GTF3C2 transfected 293T cell line lysate
Lane 2 : Non-transfected lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 101 kDa
Observed band size: 125 kDa why is the actual band size different from the predicted? -
ab89113, at 10 µg/ml staining TFIIIC110 in HeLa cells by Immunofluorescence.
实验方案
数据表及文件
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Datasheet download
文献 (3)
ab89113 被引用在 3 文献中.
- Ferrari R et al. TFIIIC Binding to Alu Elements Controls Gene Expression via Chromatin Looping and Histone Acetylation. Mol Cell 77:475-487.e11 (2020). PubMed: 31759822
- Khattar E et al. Telomerase reverse transcriptase promotes cancer cell proliferation by augmenting tRNA expression. J Clin Invest 126:4045-4060 (2016). PubMed: 27643433
- Wang J et al. Cytoskeletal Filamin A Differentially Modulates RNA Polymerase III Gene Transcription in Transformed Cell Lines. J Biol Chem 291:25239-25246 (2016). PubMed: 27738102