Anti-GTF3A抗体(ab76894)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to GTF3A
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human GTF3A.

    (Peptide available as ab90596.)

  • 阳性对照
    • This antibody gave a positive signal in human testis and liver tissue lysates as well as the following whole cell lysates: HeLa; Jurkat; HepG2.

性能

应用

Our Abpromise guarantee covers the use of ab76894 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 41 kDa).

靶标

  • 功能
    Interacts with the internal control region (ICR) of approximately 50 bases within the 5S RNA genes, is required for correct transcription of these genes by RNA polymerase III. Also binds the transcribed 5S RNA's. May initiate transcription of the 5S ribosomal RNA gene and maintain the stability of transcription of other genes.
  • 组织特异性
    Ubiquitous.
  • 序列相似性
    Contains 9 C2H2-type zinc fingers.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AP 2 antibody
    • AP2 antibody
    • Factor A antibody
    • General transcription factor 3A antibody
    • General transcription factor IIIA antibody
    • GTF 3A antibody
    • GTF3A antibody
    • TF3A_HUMAN antibody
    • TFIIIA antibody
    • Transcription factor 3A antibody
    • Transcription factor IIIA antibody
    see all

图片

  • All lanes : Anti-GTF3A antibody (ab76894) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 4 : Human liver tissue lysate - total protein (ab29889)
    Lane 5 : Human testis tissue lysate - total protein (ab30257)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 41 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 20 kDa,45 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 1 minute

实验方案

文献

ab76894 has not yet been referenced specifically in any publications.

客户评价及客户问答

Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Non-reduced Denaturing
Sample
Sheep Cell lysate - whole cell (ovine B cell line)
Specification
ovine B cell line
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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提交于 Feb 20 2015

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