The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 106 kDa (predicted molecular weight: 106 kDa).
功能May be a transcription regulator involved in cell-cycle progression and skeletal muscle differentiation. May repress GTF2I transcriptional functions, by preventing its nuclear residency, or by inhibiting its transcriptional activation. May contribute to slow-twitch fiber type specificity during myogenesis and in regenerating muscles. Binds troponin I slow-muscle fiber enhancer (USE B1). Binds specifically and with high affinity to the EFG sequences derived from the early enhancer of HOXC8.
组织特异性Highly expressed in adult skeletal muscle, heart, fibroblast, bone and fetal tissues. Expressed at lower levels in all other tissues tested.
疾病相关Note=GTF2IRD1 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Haploinsufficiency of GTF2IRD1 may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in the disease.
序列相似性Belongs to the TFII-I family. Contains 5 GTF2I-like repeats.
发展阶段Highly expressed in developing and regenerating muscles, at the time of myofiber diversification.
结构域The N-terminal half may have an activating activity.
General transcription factor II I repeat domain containing protein 1 antibody
General transcription factor II-I repeat domain-containing protein 1 antibody
General transcription factor III antibody
GTF2I repeat domain containing protein 1 antibody
GTF2I repeat domain-containing protein 1 antibody
Muscle TFII I repeat domain-containing protein 1 antibody
Muscle TFII-I repeat domain-containing protein 1 antibody
Slow muscle fiber enhancer binding protein antibody
Slow-muscle-fiber enhancer-binding protein antibody
USE B1 binding protein antibody
USE B1-binding protein antibody
Williams Beuren syndrome chromosome region 11 protein antibody
Williams-Beuren syndrome chromosomal region 11 protein antibody
Williams-Beuren syndrome chromosomal region 12 protein antibody
Anti-GTF2IRD1 antibody 图像
Western blot - GTF2IRD1 antibody (ab64805)
All lanes : Anti-GTF2IRD1 antibody (ab64805) at 1/500 dilution
Lane 1 : Extracts from 293 cells, no peptide Lane 2 : Extracts from 293 cells, plus peptide
Lysates/proteins at 5 µg per lane.
Predicted band size : 106 kDa Observed band size : 106 kDa
Anti-GTF2IRD1 antibody (ab64805)参考文献
This product has been referenced in:
Masuda T et al. The transcription factor GTF2IRD1 regulates the topology and function of photoreceptors by modulating photoreceptor gene expression across the retina. J Neurosci34:15356-68 (2014).
WB, IP, IHC
Read more (PubMed: 25392503) »