Overlay histogram showing HeLa cells stained with ab55199 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55199, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
Immunofluorescence - GTF2H1 antibody (ab55199)
GTF2H1 antibody (ab55199) used in immunofluorescence at 1-5ug/ml on HeLa cells.
IHC-P - GTF2H1 antibody (ab55199)
GTF2H1 antibody (ab55199) used in immunohistochemistry at 5ug/ml on formalin fixed and paraffin embedded human testis tissue.
Western blot - GTF2H1 antibody (ab55199)
GTF2H1 antibody (ab55199) at 1ug/lane + Jurkat cell lysate at 25ug/lane.
Anti-GTF2H1 antibody (ab55199)参考文献
This product has been referenced in:
Kainulainen M et al. Virulence factor NSs of rift valley fever virus recruits the F-box protein FBXO3 to degrade subunit p62 of general transcription factor TFIIH. J Virol88:3464-73 (2014).
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