Anti-GST3 / GST pi抗体(ab53943)


  • 产品名称Anti-GST3 / GST pi抗体
    参阅全部 GST3 / GST pi 一抗
  • 描述
    山羊多克隆抗体to GST3 / GST pi
  • 特异性ab53943 is specific for GST pi.
  • 经测试应用适用于: WB, IHC-P, IP, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Rabbit, Hamster, Human
  • 免疫原

    Full length native protein (purified) GST3/ GST pi (Rat)

  • 阳性对照
    • This antibody gave a positive result in IF in the following Formaldehyde fixed cell line: HCT116.



Our Abpromise guarantee covers the use of ab53943 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/3000. Detects a band of approximately 31 kDa (predicted molecular weight: 23 kDa).
IHC-P 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP Use at an assay dependent concentration. PubMed: 23741294
ICC/IF 1/200.


  • 功能Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.
  • 序列相似性Belongs to the GST superfamily. Pi family.
    Contains 1 GST C-terminal domain.
    Contains 1 GST N-terminal domain.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Deafness antibody
    • Deafness X-linked 7 antibody
    • DFN7 antibody
    • FAEES3 antibody
    • Fatty Acid Ethyl Ester Synthase III antibody
    • Glutathione S Transferase 3 antibody
    • Glutathione S Transferase Pi antibody
    • Glutathione S-transferase P antibody
    • Glutathione S-transferase pi 1 antibody
    • GST class-pi antibody
    • GST3 antibody
    • GSTP antibody
    • Gstp1 antibody
    • GSTP1-1 antibody
    • GSTP1_HUMAN antibody
    • PI antibody
    • X linked 7 antibody
    see all

Anti-GST3 / GST pi antibody 图像

  • IHC image of ab53943 staining in human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab53943, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Predicted band size : 23 kDa
  • ab53943 stained HCT116 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab53943 at 1/200 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 donkey anti- goat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-GST3 / GST pi antibody (ab53943)参考文献

This product has been referenced in:
  • Klaus A  et al. Glutathione S-Transferases Interact with AMP-Activated Protein Kinase: Evidence for S-Glutathionylation and Activation In Vitro. PLoS One 8:e62497 (2013). IP ; Rat . Read more (PubMed: 23741294) »

See 1 Publication for this product

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (brain)
Loading amount 5 µg
Specification brain
Gel Running Conditions Reduced Denaturing (12% SDS-PAGE)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

提交于 Dec 15 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Glioblastoma cell line)
Loading amount 20 µg
Specification Glioblastoma cell line
Treatment Lysis buffer (urea/thiourea)
Gel Running Conditions Reduced Denaturing (4-20% 1mm)
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C

Abcam user community

Verified customer

提交于 Feb 05 2010