Anti-GST3 / GST pi抗体(ab53943)

概述

  • 产品名称
    Anti-GST3 / GST pi抗体
    参阅全部 GST3 / GST pi 一抗
  • 描述
    山羊多克隆抗体to GST3 / GST pi
  • 特异性
    ab53943 is specific for GST pi.
  • 经测试应用
    适用于: WB, IHC-P, IP, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Rabbit, Hamster, Human
  • 免疫原

    Full length native protein (purified) GST3/ GST pi (Rat)

  • 阳性对照
    • This antibody gave a positive result in IF in the following Formaldehyde fixed cell line: HCT116.

性能

应用

Our Abpromise guarantee covers the use of ab53943 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/3000. Detects a band of approximately 31 kDa (predicted molecular weight: 23 kDa).
IHC-P 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP Use at an assay dependent concentration. PubMed: 23741294
ICC/IF 1/200.

靶标

  • 功能
    Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.
  • 序列相似性
    Belongs to the GST superfamily. Pi family.
    Contains 1 GST C-terminal domain.
    Contains 1 GST N-terminal domain.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Deafness antibody
    • Deafness X-linked 7 antibody
    • DFN7 antibody
    • FAEES3 antibody
    • Fatty Acid Ethyl Ester Synthase III antibody
    • Glutathione S Transferase 3 antibody
    • Glutathione S Transferase Pi antibody
    • Glutathione S-transferase P antibody
    • Glutathione S-transferase pi 1 antibody
    • GST class-pi antibody
    • GST3 antibody
    • GSTP antibody
    • Gstp1 antibody
    • GSTP1-1 antibody
    • GSTP1_HUMAN antibody
    • PI antibody
    • X linked 7 antibody
    see all

Anti-GST3 / GST pi antibody 图像

  • IHC image of ab53943 staining in human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab53943, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.



  • Predicted band size : 23 kDa
  • ab53943 stained HCT116 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab53943 at 1/200 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 donkey anti- goat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-GST3 / GST pi antibody (ab53943)参考文献

This product has been referenced in:
  • Cen J  et al. Long-Term Alteration of Reactive Oxygen Species Led to Multidrug Resistance in MCF-7 Cells. Oxid Med Cell Longev 2016:7053451 (2016). Read more (PubMed: 28058088) »
  • Raposo C  et al. Sildenafil (Viagra) protective effects on neuroinflammation: the role of iNOS/NO system in an inflammatory demyelination model. Mediators Inflamm 2013:321460 (2013). WB . Read more (PubMed: 23970812) »

See all 3 Publications for this product

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (brain)
Loading amount
5 µg
Specification
brain
Gel Running Conditions
Reduced Denaturing (12% SDS-PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Dec 15 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Glioblastoma cell line)
Loading amount
20 µg
Specification
Glioblastoma cell line
Treatment
Lysis buffer (urea/thiourea)
Gel Running Conditions
Reduced Denaturing (4-20% 1mm)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Feb 05 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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