Anti-GST3 / GST pi抗体(ab36149)

概述

  • 产品名称
    Anti-GST3 / GST pi抗体
    参阅全部 GST3 / GST pi 一抗
  • 描述
    山羊多克隆抗体to GST3 / GST pi
  • 宿主
    Goat
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow, Dog
  • 免疫原

    Synthetic peptide:

    LADQGQSWKEEV

    , corresponding to Internal sequence amino acids 21-32 of Human GST3

  • 阳性对照
    • Human kidney lysates. Lysate prepared from transfected HEK293 cells transiently expressing GST3 / GST pi.

性能

应用

Our Abpromise guarantee covers the use of ab36149 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 23 kDa).

靶标

图片

  • Anti-GST3 / GST pi antibody (ab36149) at 0.01 µg/ml + Human Kidney lysate (35µg protein in RIPA buffer).

    Predicted band size: 23 kDa
    Observed band size: 23 kDa



    Primary incubation was 1 hour. Detected by chemiluminescence.
  • Anti-GST3 / GST pi antibody (ab36149) at 0.03 µg/ml + lysate prepared from transfected HEK293 cells transiently expressing GST3 / GST pi

    Predicted band size: 23 kDa
    Observed band size: 26 kDa (why is the actual band size different from the predicted?)

实验方案

文献

This product has been referenced in:
  • Cooper TK  et al. Use of urinary biomarkers of renal ischemia in a lamb preclinical left ventricular assist device model. Artif Organs 36:820-4 (2012). Sheep . Read more (PubMed: 22428807) »

See 1 Publication for this product

客户评价及客户问答

Application
Western blot
Sample
Mouse Tissue lysate - whole (Eye retinal protein)
Loading amount
50 µg
Specification
Eye retinal protein
Gel Running Conditions
Reduced Denaturing (4-14%)
Blocking step
BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

提交于 Dec 30 2011

DISCOUNT CODE: *** Expiration date: April 21st, 2012 I am very pleased to hear you would like to accept our offer and test ab36149 in IHC. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit a...

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Thank you for your enquiry. I am sorry I did not mention this earlier but I can offer you a discount of 15% off the order of ab4606, normal mouse kidney slides: http://www.abcam.com/Kidney-mouse-normal-tissue-slides-ab4606.html Include this discount co...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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