Anti-GST3 / GST pi抗体(ab106268)

概述

  • 产品名称Anti-GST3 / GST pi抗体
    参阅全部 GST3 / GST pi 一抗
  • 描述
    兔多克隆抗体to GST3 / GST pi
  • 经测试应用适用于: WB, IHC-P, ICCmore details
  • 种属反应性
    与反应: Rat, Human
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide mapping to the C terminus of Human Glutathione S Transferase pi, differing from the related Mouse sequence by two amino acids.

  • 阳性对照
    • HeLa cell lysate, Endometrial carcinoma section This antibody gave a positive result in IF/ICC when used in the following formaldehyde fixed cell lines: A549.

性能

应用

Our Abpromise guarantee covers the use of ab106268 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 23 kDa.
IHC-P Use a concentration of 1 - 2 µg/ml. Boiling the sections is required.
ICC Use a concentration of 0.5 - 1 µg/ml. In acetone fixed cell. Antigen retrieval by Pepsin and Trypsin is required.

靶标

  • 功能Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.
  • 序列相似性Belongs to the GST superfamily. Pi family.
    Contains 1 GST C-terminal domain.
    Contains 1 GST N-terminal domain.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Deafness antibody
    • Deafness X-linked 7 antibody
    • DFN7 antibody
    • FAEES3 antibody
    • Fatty Acid Ethyl Ester Synthase III antibody
    • Glutathione S Transferase 3 antibody
    • Glutathione S Transferase Pi antibody
    • Glutathione S-transferase P antibody
    • Glutathione S-transferase pi 1 antibody
    • GST class-pi antibody
    • GST3 antibody
    • GSTP antibody
    • Gstp1 antibody
    • GSTP1-1 antibody
    • GSTP1_HUMAN antibody
    • PI antibody
    • X linked 7 antibody
    see all

Anti-GST3 / GST pi antibody 图像

  • Anti-GST3 / GST pi antibody (ab106268) at 2 µg/ml + HeLa cell lysate

    Predicted band size : 23 kDa
  • Cytoplasmic staining of Glutathione S Transferase pi in a formalin fixed, paraffin embedded section of endometrial carcinoma, using ab106268 at a concentration of 2 µg/ml.
  • ICC/IF image of ab106268 stained A549 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab106268 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-GST3 / GST pi antibody (ab106268)参考文献

ab106268 has not yet been referenced specifically in any publications.

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