概述

  • 产品名称Anti-GST抗体(HRP)
    参阅全部 GST 一抗
  • 描述
    山羊多克隆抗体to GST (HRP)
  • 偶联物HRP
  • 经测试应用适用于: IP, ELISA, WB, IHC-Pmore details
  • 免疫原

    Glutathione-S-Transferase [Schistosoma japonicum]

性能

应用

Our Abpromise guarantee covers the use of ab58626 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at an assay dependent concentration. PubMed: 24623412

Works even though HRP conjugated.

ELISA 1/20000.
WB 1/2000 - 1/5000.
IHC-P 1/500 - 1/2000.

靶标

  • 相关性GST (Glutathione S-Transferase) is a 26kDa protein encoded by the parasitic helminth Schistosoma japonicum and widely used in the pGEX family of GST plasmid expression vectors as a fusion protein with foreign proteins.
  • 别名
    • Glutathione S Transferase antibody
    • Glutathione S-transferase class-mu 26 kDa isozyme antibody
    • GST antibody
    • Sj26 antigen antibody
    • SjGST antibody
    see all

Anti-GST antibody (HRP) 图像

  • ab58626 staining GST in by ELISA. The sample was blocked in 1% milk for 1 hour at 18°C. The primary was diluted at 1/2000 in PBS-Tween20 buffer and incubated with sample for 1 hour at 18°C.

    See Abreview

Anti-GST antibody (HRP) (ab58626)参考文献

This product has been referenced in:
  • Panas MD  et al. The C-terminal repeat domains of nsP3 from the Old World alphaviruses bind directly to G3BP. J Virol 88:5888-93 (2014). IP . Read more (PubMed: 24623412) »
  • Castillo-Lluva S  et al. SUMOylation of the GTPase Rac1 is required for optimal cell migration. Nat Cell Biol 12:1078-85 (2010). WB ; Human . Read more (PubMed: 20935639) »

See all 3 Publications for this product

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Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application ELISA
Sample Rat Purified protein (smooth muscle)
Specification smooth muscle
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 18°C
Type Indirect
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Abcam user community

Verified customer

提交于 May 06 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Escherichia coli Recombinant protein (Expressed in E.Coli)
Loading amount 10 µg
Specification Expressed in E.Coli
Gel Running Conditions Reduced Denaturing (PVDF)
Blocking step Block-it PBS-T as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 21°C
Username

Abcam user community

Verified customer

提交于 Mar 27 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"