概述

  • 产品名称
    Anti-GST抗体[EPR4236]
    参阅全部 GST 一抗
  • 描述
    兔单克隆抗体[EPR4236] to GST
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WB, ICC/IF, IPmore details
    不适用于: Flow Cyt or IHC-P
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) (GST-tag) corresponding to GST aa 300-450. GST tag was fused to ATG16L1 to produce a recombinant protein.
    Database link: Q676U5

  • 阳性对照
    • GST fusion proteins; Alpha-actinin-4-GST, AKT1/PKBa-GST or CDK7-GST.
  • 常规说明

    A trial size is available to purchase for this antibody.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

性能

应用

Our Abpromise guarantee covers the use of ab111947 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 26 kDa.
ICC/IF 1/100.
IP 1/20.
  • 应用说明
    Is unsuitable for Flow Cyt or IHC-P.

    • 靶标

    • 相关性
      GST (Glutathione S-Transferase) is a 26kDa protein encoded by the parasitic helminth Schistosoma japonicum and widely used in the pGEX family of GST plasmid expression vectors as a fusion protein with foreign proteins.
    • 别名
      • Glutathione S Transferase antibody
      • Glutathione S-transferase class-mu 26 kDa isozyme antibody
      • GST antibody
      • Sj26 antigen antibody
      • SjGST antibody
      see all

    图片

    • Immunocytochemistry/ Immunofluorescence - Anti-GST antibody [EPR4236] (ab111947)
      Immunocytochemistry/ Immunofluorescence - Anti-GST antibody [EPR4236] (ab111947)

      Immunofluorescence staining of 293T cells transfected with GST, with ab111947 at a working dilution of 1/100 and counter-stained with DAPI. Tubulin was also stained with mouse anti-tubulin at a dilution of 1/1000 (ab7291) and Alexa Fluor® 594 goat anti-mouse at a dilution of 1/500 (ab150120). The secondary antibody was Alexa Fluor® 488 goat anti rabbit (ab150077), used at a dilution of 1/500. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X-100. The negative controls are shown in the bottom hand panels - for the first negative control, anti-GST antibody (ab111947) was used at a dilution of 1/100 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/1000 and for the second negative control, mouse anti-tubulin (ab7291) and anti-rabbit secondary antibody (ab150077) were used.

    • Immunoprecipitation - Anti-GST antibody [EPR4236] (ab111947)
      Immunoprecipitation - Anti-GST antibody [EPR4236] (ab111947)

      GST immunoprecipitation from transfected 293T whole cell lysate using ab111947. 1 mg of cell lysate was incubated with capture antibody (ab111947) at 1/20 dilution.

      Lane 1: Primary: ab111947, 1/1000 dilution
      Sample: 293T transfected with GST tagged protein vector cell lysate 10 ug
      Lane 2: Primary: ab111947, 1/1000 dilution
      Sample: 293T transfected with GST tagged protein vector cell lysate
      Lane 3: Primary: ab172730, Rabbit monoclonal IgG
      Sample: 293T transfected with GST tagged protein vector cell lysate

      All lanes: Secondary: ab131366, Veriblot, 1/10000 dilution
      Blocking and diluting buffer: 5% NFDM/TBST



      Lanes 1-2 : Anti-GST antibody [EPR4236] (ab111947) at 1/1000 dilution
      Lane 3 : Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

      Lane 1 : Input - 293 transfected with GST tagged protein vector cell lysate at 10 µg
      Lane 2 : (+) 293T transfected with GST tagged protein vector cell lysate
      Lane 3 : (-) 293T transfected with GST tagged protein vector cell lysate

      Secondary
      All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/10000 dilution (VeriBlot)

      Additional bands at: 41 kDa (possible tagged protein)

    • Western blot - GST antibody [EPR4236] (ab111947)
      Western blot - GST antibody [EPR4236] (ab111947)
      Anti-GST antibody [EPR4236] (ab111947) at 1/1000 dilution + GST recombinant protein at 0.01 µg

      Predicted band size: 26 kDa

    • Immunoprecipitation - Anti-GST antibody [EPR4236] (ab111947)
      Immunoprecipitation - Anti-GST antibody [EPR4236] (ab111947)

      (Cross IP) GST immunoprecipitation from transfected 293T whole cell lysate using ab111947. 1 mg of cell lysate was incubated with capture antibody (ab111947) at 1/20 dilution.

      Lane 1: Primary: ab180123, 1/1000 dilution
      Sample: 293T transfected with GST tagged protein vector cell lysate 10 ug
      Lane 2: Primary: ab180123, 1/1000 dilution
      Sample: 293T transfected with GST tagged protein vector cell lysate
      Secondary: ab131366, Veriblot, 1/10000 dilution
      Lane 3: Primary: ab172730, Rabbit monoclonal IgG
      Sample: 293T transfected with GST tagged protein vector cell lysate

      All lanes: Secondary: ab131366, Veriblot, 1/10000 dilution
      Blocking and diluting buffer: 5% NFDM/TBST

    • Western blot - GST antibody [EPR4236] (ab111947)
      Western blot - GST antibody [EPR4236] (ab111947)
      All lanes : Anti-GST antibody [EPR4236] (ab111947) at 1/1000 dilution

      Lane 1 : Alpha-actinin-4-GST fusion protein
      Lane 2 : AKT1/PKBa-GST fusion protein
      Lane 3 : CDK7-GST fusion protein

      Lysates/proteins at 0.01 µg per lane.

      Predicted band size: 26 kDa

    文献

    This product has been referenced in:
    • Kondo Y  et al. Sensitive detection of fluorescence in western blotting by merging images. PLoS One 13:e0191532 (2018). WB ; Mouse . Read more (PubMed: 29352284) »
    • Wang J  et al. Identification of candidate substrates of ubiquitin-specific protease 13 using 2D-DIGE. Int J Mol Med 40:47-56 (2017). WB ; Human . Read more (PubMed: 28498477) »

    See all 3 Publications for this product

    发表研究结果有使用 ab111947？请让我们知道，以便我们可以引用本数据表中的参考文章。

    客户评价及客户问答

    提交评价 提交问题

    There are currently no Customer reviews or Questions for ab111947.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    注册