Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) (GST-tag) corresponding to GST aa 300-450. GST tag was fused to ATG16L1 to produce a recombinant protein.
Database link: Q676U5
A trial size is available to purchase for this antibody.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Our Abpromise guarantee covers the use of ab111947 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用 | Ab评论 | 说明 |
---|---|---|
WB | 1/1000 - 1/10000. Predicted molecular weight: 26 kDa. | |
ICC/IF | 1/100. | |
IP | 1/20. |
Immunofluorescence staining of 293T cells transfected with GST, with ab111947 at a working dilution of 1/100 and counter-stained with DAPI. Tubulin was also stained with mouse anti-tubulin at a dilution of 1/1000 (ab7291) and Alexa Fluor® 594 goat anti-mouse at a dilution of 1/500 (ab150120). The secondary antibody was Alexa Fluor® 488 goat anti rabbit (ab150077), used at a dilution of 1/500. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X-100. The negative controls are shown in the bottom hand panels - for the first negative control, anti-GST antibody (ab111947) was used at a dilution of 1/100 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/1000 and for the second negative control, mouse anti-tubulin (ab7291) and anti-rabbit secondary antibody (ab150077) were used.
GST immunoprecipitation from transfected 293T whole cell lysate using ab111947. 1 mg of cell lysate was incubated with capture antibody (ab111947) at 1/20 dilution.
Lane 1: Primary: ab111947, 1/1000 dilution
Sample: 293T transfected with GST tagged protein vector cell lysate 10 ug
Lane 2: Primary: ab111947, 1/1000 dilution
Sample: 293T transfected with GST tagged protein vector cell lysate
Lane 3: Primary: ab172730, Rabbit monoclonal IgG
Sample: 293T transfected with GST tagged protein vector cell lysate
All lanes: Secondary: ab131366, Veriblot, 1/10000 dilution
Blocking and diluting buffer: 5% NFDM/TBST
(Cross IP) GST immunoprecipitation from transfected 293T whole cell lysate using ab111947. 1 mg of cell lysate was incubated with capture antibody (ab111947) at 1/20 dilution.
Lane 1: Primary: ab180123, 1/1000 dilution
Sample: 293T transfected with GST tagged protein vector cell lysate 10 ug
Lane 2: Primary: ab180123, 1/1000 dilution
Sample: 293T transfected with GST tagged protein vector cell lysate
Secondary: ab131366, Veriblot, 1/10000 dilution
Lane 3: Primary: ab172730, Rabbit monoclonal IgG
Sample: 293T transfected with GST tagged protein vector cell lysate
All lanes: Secondary: ab131366, Veriblot, 1/10000 dilution
Blocking and diluting buffer: 5% NFDM/TBST
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"