概述

  • 产品名称Anti-GST抗体[1E5]
    参阅全部 GST 一抗
  • 描述
    小鼠单克隆抗体[1E5] to GST
  • 经测试应用适用于: ICC/IF, ELISA, WBmore details
  • 种属反应性
    not applicable
  • 免疫原

    Recombinant GST purified from E. coli

  • 阳性对照
    • recombinant GST and GST fusion protein

性能

应用

Our Abpromise guarantee covers the use of ab69186 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/100.
ELISA Use at an assay dependent concentration.
WB 1/500 - 1/2000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).

靶标

  • 相关性GST (Glutathione S-Transferase) is a 26kDa protein encoded by the parasitic helminth Schistosoma japonicum and widely used in the pGEX family of GST plasmid expression vectors as a fusion protein with foreign proteins.
  • 别名
    • Glutathione S Transferase antibody
    • Glutathione S-transferase class-mu 26 kDa isozyme antibody
    • GST antibody
    • Sj26 antigen antibody
    • SjGST antibody
    see all

Anti-GST antibody [1E5] 图像

  • Immunofluorescence analysis of GST in HeLa cells using ab69186 at 1/100. Cells were counter-stained with DAPI (blue) for nuclear staining and a Alexa Fluor® 488 conjugated goat anti-mouse IgG was used as the secondary antibody (green).

  • All lanes : Anti-GST antibody [1E5] (ab69186) at 1/1000 dilution

    Lane 1 : GST recombinant protein
    Lane 2 : GST-fusion protein

    Lysates/proteins at 0.02 µg per lane.

    Secondary
    Goat anti-mouse secondary antibody conjugated to HRP
    Developed using the ECL technique

    Predicted band size : 26 kDa
    Observed band size : 26 kDa
    Additional bands at : 55 kDa (possible Fusion protein).

Anti-GST antibody [1E5] (ab69186)参考文献

ab69186 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab69186.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"