GST Activity Assay试剂盒(Fluorometric) (ab65325)

概述

  • 产品名称GST Activity Assay试剂盒(Fluorometric)
    参阅全部 GST 试剂盒
  • 测试
    100 x 1 assay
  • 样品类型
    Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate
  • 检测类型Enzyme activity
  • 灵敏度
    < 0.5 mU/well
  • 检测时间
    2h 00m
  • 产品概述

    Abcam's GST Activity Assay Kit (Fluorometric) provides a simple, fluorescence-based in vitro assay for detecting the GST activity using fluorescence plate reader. The assay utilizes monochlorobimane (MCB), a dye that reacts with glutathione. The free form of MCB is almost nonfluorescent, whereas the dye fluoresces in blue (Ex/Em = 380/461 nm) after reaction with glutathione catalysed by GST. The change in fluorescence overtime allows for an easy measurement of the sample GST level.

    The kit can detect GST activity in crude cell lysate or purified protein fraction, and also quantitate GST-tagged fusion protein.
    Visit our FAQs page for tips and troubleshooting.

  • 说明

    Glutathione S-transferase (GST) is a family of enzymes that play an important role in detoxification of xenobiotics. GST catalyzes the formation of the thiol group of glutathione to electrophilic xenobiotics. It utilizes glutathione to scavenge potentially toxic compounds including those produced as a result of oxidative stress and is part of the defense mechanism against the mutagenic, carcinogenic and toxic effects of such compounds.

  • 经测试应用适用于: Functional Studiesmore details

性能

  • 相关性Glutathione S-transferase (GST) is a family of enzymes that play an important role in detoxification of xenobiotics. GST catalyzes the formation of the thiol group of glutathione to electrophilic xenobiotics. It utilizes glutathione to scavenge potentially toxic compounds including those produced as a result of oxidative stress and is part of the defense mechanism against the mutagenic, carcinogenic and toxic effects of such compounds. Based on their biochemical, immunological, and structural properties, the soluble human GSTs are categorized into 4 main classes: alpha, mu, pi, and theta.
  • 细胞定位Cytoplasmic
  • 别名
    • Glutathione S transferase A1
    • Glutathione S transferase A2
    • GST class alpha 1
    • GST class alpha 2
    • GST epsilon
    • GST gamma
    • GSTA1
    • GSTA2
    • GTH1
    • GTH2
    • HA subunit 1
    • HA subunit 2
    see all

应用

Our Abpromise guarantee covers the use of ab65325 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Functional Studies Use at an assay dependent dilution.

GST Activity Assay Kit (Fluorometric) 图像

  • Standard Calibration Curve of GST Measured by Fluorometry. Various amounts of standard GST were incubated with GSH and MCB according to the kit instructions. Fluorescence was measured at ex/em 380/460 nm.

实验方案

GST Activity Assay Kit (Fluorometric) (ab65325)参考文献

ab65325 has not yet been referenced specifically in any publications.

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