Anti-GSK3 beta (phospho Y216) + GSK3 alpha (phospho Y279)抗体(ab75745)
Key features and details
- Rabbit polyclonal to GSK3 beta (phospho Y216) + GSK3 alpha (phospho Y279)
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-GSK3 beta (phospho Y216) + GSK3 alpha (phospho Y279)抗体 -
描述
兔多克隆抗体to GSK3 beta (phospho Y216) + GSK3 alpha (phospho Y279) -
宿主
Rabbit -
特异性
ab75745 detects endogenous levels of GSK3 beta only when phosphorylated at tyrosine 216 and GSK3 alpha when phosphorylated at tyrosine 276. This antibody can also cross-react with GSK3 alpha subunit due to the consistent antigen-sequence of "GEPNVSYICSRYY".
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经测试应用
适用于: ICC/IF, WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
This product was produced with the following immunogens:
Synthetic peptide corresponding to Human GSK3 beta (phospho Y216).
Synthetic peptide corresponding to Human GSK3 alpha (phospho Y279).
Database link: P49840 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.87% Sodium chloride, 50% Glycerol, PBS
Without Mg2+ and Ca2+ -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
ab75745 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab75745于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF |
1/100 - 1/200.
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WB | (4) |
1/500 - 1/1000. Predicted molecular weight: 47 kDa. This antibody can be used with studies using ab110532.
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IHC-P |
1/50 - 1/100.
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说明 |
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ICC/IF
1/100 - 1/200. |
WB
1/500 - 1/1000. Predicted molecular weight: 47 kDa. This antibody can be used with studies using ab110532. |
IHC-P
1/50 - 1/100. |
靶标
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细胞定位
GSK3 beta: Cytoplasm. Nucleus. Cell membrane. The phosphorylated form shows localization to cytoplasm and cell membrane. The MEMO1-RHOA-DIAPH1 signaling pathway controls localization of the phosophorylated form to the cell membrane. -
数据库链接
- Entrez Gene: 2931 Human
- Entrez Gene: 2932 Human
- Entrez Gene: 56637 Mouse
- Entrez Gene: 606496 Mouse
- Entrez Gene: 50686 Rat
- Entrez Gene: 84027 Rat
- Omim: 605004 Human
- Omim: 606784 Human
see all
图片
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All lanes : Anti-GSK3 beta (phospho Y216) + GSK3 alpha (phospho Y279) antibody (ab75745) at 1/500 dilution
Lane 1 : 293 cell extracts, untreated
Lane 2 : 293 cell extracts, treated with insulin (40µM, 30min)
Predicted band size: 47 kDa
Observed band size: 47 kDa -
ab75745, at a 1/50 dilution, staining GSK3 alpha/beta in paraffin embedded human breast carcinoma tissue by Immunohistochemistry, in the absence (left image) or presence (right image) of the immunizing phospho peptide.
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ab75745 at 1/100 dilution staining GSK3 alpha/beta in human Hela cells by ICC/IF.
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All lanes : Anti-GSK3 beta (phospho Y216) + GSK3 alpha (phospho Y279) antibody (ab75745) at 1/500 dilution
Lane 1 : 293 cells untreated PMA
Lane 2 : 293 cells treated PMA
Predicted band size: 47 kDa
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (153)
ab75745 被引用在 153 文献中.
- Liu JJ et al. Upregulation of TRPM8 can promote the colon cancer liver metastasis through mediating Akt/GSK-3 signal pathway. Biotechnol Appl Biochem 69:230-239 (2022). PubMed: 33432591
- Kim KI et al. Sorafenib induces pigmentation via the regulation of β-catenin signalling pathway in melanoma cells. Exp Dermatol 31:57-63 (2022). PubMed: 32391926
- Lee HJ et al. Effects of electroacupuncture on the functionality of NG2-expressing cells in perilesional brain tissue of mice following ischemic stroke. Neural Regen Res 17:1556-1565 (2022). PubMed: 34916441
- Shi YL et al. Trilobatin, a Natural Food Additive, Exerts Anti-Type 2 Diabetes Effect Mediated by Nrf2/ARE and IRS-1/GLUT2 Signaling Pathways. Front Pharmacol 13:828473 (2022). PubMed: 35153796
- Bukovac A et al. Bilateral Meningioma: A Case Report and Review of the Literature. Int J Mol Sci 23:N/A (2022). PubMed: 35163107