Anti-GSK3 beta (phospho S9)抗体(ab118885)

概述

  • 产品名称Anti-GSK3 beta (phospho S9)抗体
    参阅全部 GSK3 beta 一抗
  • 描述
    兔多克隆抗体to GSK3 beta (phospho S9)
  • 经测试应用适用于: ELISA, WB, IP, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic phospho peptide:

    RPRTTSFAE

    conjugated to KLH, corresponding to amino acids 4-12 of GSK3 beta.

  • 阳性对照
    • Human ovary tissue.

应用

Our Abpromise guarantee covers the use of ab118885 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/10000 - 1/50000.
EMSA Use at an assay dependent concentration.
WB 1/500 - 1/3000. Predicted molecular weight: 47 kDa.
IP Use at an assay dependent concentration.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/200 - 1/1000.

靶标

  • 功能Participates in the Wnt signaling pathway. Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. Phosphorylates MUC1 in breast cancer cells, and decreases the interaction of MUC1 with CTNNB1/beta-catenin. Phosphorylates CTNNB1/beta-catenin. Phosphorylates SNAI1. Plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. Prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization. Phosphorylates MACF1 and this phosphorylation inhibits the binding of MACF1 to microtubules which is critical for its role in bulge stem cell migration and skin wound repair.
  • 组织特异性Expressed in testis, thymus, prostate and ovary and weakly expressed in lung, brain and kidney.
  • 序列相似性Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily.
    Contains 1 protein kinase domain.
  • 翻译后修饰Phosphorylated by AKT1 and ILK1. Activated by phosphorylation at Tyr-216.
  • 细胞定位Cytoplasm. Nucleus. Cell membrane. The phosphorylated form shows localization to cytoplasm and cell membrane. The MEMO1-RHOA-DIAPH1 signaling pathway controls localization of the phosophorylated form to the cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Glycogen Synthase Kinase 3 Beta antibody
    • Glycogen synthase kinase-3 beta antibody
    • GSK 3 beta antibody
    • GSK-3 beta antibody
    • GSK3B antibody
    • GSK3B_HUMAN antibody
    • GSK3beta isoform antibody
    • Serine/threonine-protein kinase GSK3B antibody
    see all

Anti-GSK3 beta (phospho S9) antibody 图像

  • ab118885, at 5 µg/ml, staining GSK3 beta in Formalin-fixed, Paraffin-embedded Human Ovary tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Anti-GSK3 beta (phospho S9) antibody (ab118885)参考文献

ab118885 has not yet been referenced specifically in any publications.

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