Anti-GSK3 beta抗体(ab131356)
Key features and details
- Rabbit polyclonal to GSK3 beta
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-GSK3 beta抗体
参阅全部 GSK3 beta 一抗 -
描述
兔多克隆抗体to GSK3 beta -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide conjugated to KLH, surrounding amino acids 7-11 (
TTSFA
) of Human GSK3 beta (NP_001139628.1). -
阳性对照
- A2780 and HT29 cell extracts; HeLa cells; Human breast carcinoma tissue
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
PBS is without Mg2+ and Ca2+ -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
ab131356 is purified by affinity chromatography using epitope specific peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab131356于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/500 - 1/1000. Predicted molecular weight: 48 kDa.
|
|
IHC-P |
1/50 - 1/100.
|
|
ICC/IF |
1/100 - 1/200.
|
说明 |
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WB
1/500 - 1/1000. Predicted molecular weight: 48 kDa. |
IHC-P
1/50 - 1/100. |
ICC/IF
1/100 - 1/200. |
靶标
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功能
Participates in the Wnt signaling pathway. Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. Phosphorylates MUC1 in breast cancer cells, and decreases the interaction of MUC1 with CTNNB1/beta-catenin. Phosphorylates CTNNB1/beta-catenin. Phosphorylates SNAI1. Plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. Prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization. Phosphorylates MACF1 and this phosphorylation inhibits the binding of MACF1 to microtubules which is critical for its role in bulge stem cell migration and skin wound repair. -
组织特异性
Expressed in testis, thymus, prostate and ovary and weakly expressed in lung, brain and kidney. -
序列相似性
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily.
Contains 1 protein kinase domain. -
翻译后修饰
Phosphorylated by AKT1 and ILK1. Activated by phosphorylation at Tyr-216. -
细胞定位
Cytoplasm. Nucleus. Cell membrane. The phosphorylated form shows localization to cytoplasm and cell membrane. The MEMO1-RHOA-DIAPH1 signaling pathway controls localization of the phosophorylated form to the cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 2932 Human
- Entrez Gene: 56637 Mouse
- Entrez Gene: 84027 Rat
- Omim: 605004 Human
- SwissProt: P49841 Human
- SwissProt: Q9WV60 Mouse
- SwissProt: P18266 Rat
- Unigene: 445733 Human
see all -
别名
- Glycogen Synthase Kinase 3 Beta antibody
- Glycogen synthase kinase-3 beta antibody
- GSK 3 beta antibody
see all
图片
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All lanes : Anti-GSK3 beta antibody (ab131356) at 1/500 dilution
Lane 1 : A2780 cell extract
Lane 2 : HT29 cell extract
Predicted band size: 48 kDa -
Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue labelling GSK3 beta with ab131356 at a dilution of 1/50. Picture on the right was preincubated with blocking peptide.
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Immunofluorescence of methanol-fixed HeLa cells staining GSK3 beta with ab131356 at a dilution of 1/100.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (28)
ab131356 被引用在 28 文献中.
- Niu D et al. Protective Effects of Iridoid Glycoside from Corni Fructus on Type 2 Diabetes with Nonalcoholic Fatty Liver in Mice. Biomed Res Int 2021:3642463 (2021). PubMed: 33542919
- Ren WM et al. Neuroprotective Effects of ZiBuPiYin Recipe on db/db Mice via PI3K-Akt Signaling Pathway by Activating Grb2. Neural Plast 2021:8825698 (2021). PubMed: 33603781
- Wu Q et al. Tumor suppressor role of sFRP-4 in hepatocellular carcinoma via the Wnt/ß-catenin signaling pathway. Mol Med Rep 23:N/A (2021). PubMed: 33760186
- Shi H et al. Activation of Galanin Receptor 1 with M617 Attenuates Neuronal Apoptosis via ERK/GSK-3β/TIP60 Pathway After Subarachnoid Hemorrhage in Rats. Neurotherapeutics 18:1905-1921 (2021). PubMed: 34086200
- Li SZ et al. miR-139/PDE2A-Notch1 feedback circuit represses stemness of gliomas by inhibiting Wnt/β-catenin signaling. Int J Biol Sci 17:3508-3521 (2021). PubMed: 34512162