Anti-GSK3 (alpha + beta) (phospho Y279 + Y216)抗体(ab52188)


  • 产品名称Anti-GSK3 (alpha + beta) (phospho Y279 + Y216)抗体
    参阅全部 GSK3 (alpha + beta) 一抗
  • 描述
    兔多克隆抗体to GSK3 (alpha + beta) (phospho Y279 + Y216)
  • 特异性Detects endogenous levels of GSK3a/ß only when phosphorylated at tyrosine 279/216.
  • 经测试应用适用于: ICC/IF, WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic phosphopeptide derived from human GSK3a/ß around the phosphorylation site of tyrosine 279/216 (V-S-YP-I-C).

  • 阳性对照
    • Human breast carcinoma tissue, extracts from HeLa cells.




Our Abpromise guarantee covers the use of ab52188 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration. PubMed: 21261990
WB 1/500 - 1/1000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
ELISA 1/20000.
IHC-P Use at an assay dependent concentration.


  • 相关性Glycogen synthase kinase 3 (GSK3) is a proline directed serine threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase, a key enzyme in glycogen metabolism. Since then, it has been shown to be involved in the regulation of a diverse array of cellular functions, including protein synthesis, cell proliferation, cell differentiation, microtubule assembly/disassembly, and apoptosis. GSK3s substrate specificity is unique in that phosphorylation of substrate only occurs if a phosphoserine or phosphotyrosine is present four residues C terminal to the site of GSK phosphorylation. There exists two isoforms of GSK3, alpha and beta, and they show a high degree of amino acid homology. The two isoforms of GSK3 are strictly regulated via phosphorylation. Phosphorylation of GSK3 beta on Ser9 (Ser21 in GSK3 alpha) by protein kinase B (PKB) causes its inactivation is the primary mechanism responsible for growth factor inhibition of this kinase. Activation of GSK3 beta is dependent upon the phosphorylation of Tyr216 (Tyr279 in GSK3 alpha). Upon activation, it has been shown to phosphorylate a number of different cellular proteins, including p53, c-Myc, c-Jun, heat shock factor 1 (HSF1), and cyclin D1. GSK3 beta also has been shown to phosphorylate aberrant sites on the microtubule associated protein tau, which is critical for the progression of Alzheimer's disease. GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation.
  • 细胞定位Cytoplasmic and Nuclear
  • 数据库链接
  • 别名
    • Factor A antibody
    • Glycogen synthase kinase 3 alpha antibody
    • Glycogen synthase kinase 3 beta antibody
    • GSK3 alpha antibody
    • GSK3 beta antibody
    • GSK3B antibody
    see all

Anti-GSK3 (alpha + beta) (phospho Y279 + Y216) antibody 图像

  • All lanes : Anti-GSK3 (alpha + beta) (phospho Y279 + Y216) antibody (ab52188) at 1/500 dilution

    Lane 1 : extracts from HeLa cells with blocking peptide
    Lane 2 : extracts from HeLa cells

    Predicted band size : 52 kDa
    Observed band size : 51,52 kDa (why is the actual band size different from the predicted?)
  • Ab52188 at 1/50 dilution staining human breast carcinoma tissue with and without blocking peptide; paraffin embedded.

Anti-GSK3 (alpha + beta) (phospho Y279 + Y216) antibody (ab52188)参考文献

This product has been referenced in:
  • Atkinson JM  et al. Activating the Wnt/ß-Catenin Pathway for the Treatment of Melanoma--Application of LY2090314, a Novel Selective Inhibitor of Glycogen Synthase Kinase-3. PLoS One 10:e0125028 (2015). WB ; Human . Read more (PubMed: 25915038) »
  • Darrington RS  et al. Distinct expression and activity of GSK-3a and GSK-3ß in prostate cancer. Int J Cancer 131:E872-83 (2012). Read more (PubMed: 22539113) »

See all 3 Publications for this product

Product Wall

Thank you for your recent telephone enquiry. I can confirm the general IHC testing protocol used for ab52188 GSK3 (alpha + beta) (phospho Y279 + Y216) antibody by our source is as follows. As discussed on the telephone, we would recommend this as a...

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