概述

  • 产品名称Anti-GSK3 (alpha + beta)抗体
    参阅全部 GSK3 (alpha + beta) 一抗
  • 描述
    兔多克隆抗体to GSK3 (alpha + beta)
  • 经测试应用适用于: ICC, WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Chicken, Monkey
  • 免疫原

    Synthetic peptide (Human) (C terminal).

  • 阳性对照
    • Jurkat cells and brain
  • 常规说明


    GSK3 (Glycogen Synthase Kinase-3 differs from most serine/threonine kinases) in that it is active in the absence of the action of signaling pathways. Two isoforms exist, GSK-3a and GSK-3ß. The function of GSK-3 is to phosphorylate glycogen synthase and thereby inactivate it. Insulin action stimulates the PI 3-kinase pathway, resulting in Akt activation, which phosphorylates and inactivates GSK-3.

性能

应用

Our Abpromise guarantee covers the use of ab15314 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC Use at an assay dependent concentration. PubMed: 22003387
WB Use at an assay dependent concentration. Predicted molecular weight: 52 kDa.
IHC-P Use at an assay dependent concentration. 1/100 for 10 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate, pH 6.0 for 10 min followed by cooling at RT for 20 min.

靶标

  • 相关性Glycogen synthase kinase 3 (GSK3) is a proline directed serine threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase, a key enzyme in glycogen metabolism. Since then, it has been shown to be involved in the regulation of a diverse array of cellular functions, including protein synthesis, cell proliferation, cell differentiation, microtubule assembly/disassembly, and apoptosis. GSK3s substrate specificity is unique in that phosphorylation of substrate only occurs if a phosphoserine or phosphotyrosine is present four residues C terminal to the site of GSK phosphorylation. There exists two isoforms of GSK3, alpha and beta, and they show a high degree of amino acid homology. The two isoforms of GSK3 are strictly regulated via phosphorylation. Phosphorylation of GSK3 beta on Ser9 (Ser21 in GSK3 alpha) by protein kinase B (PKB) causes its inactivation is the primary mechanism responsible for growth factor inhibition of this kinase. Activation of GSK3 beta is dependent upon the phosphorylation of Tyr216 (Tyr279 in GSK3 alpha). Upon activation, it has been shown to phosphorylate a number of different cellular proteins, including p53, c-Myc, c-Jun, heat shock factor 1 (HSF1), and cyclin D1. GSK3 beta also has been shown to phosphorylate aberrant sites on the microtubule associated protein tau, which is critical for the progression of Alzheimer's disease. GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation.
  • 细胞定位Cytoplasmic and Nuclear
  • 数据库链接
  • 别名
    • Factor A antibody
    • Glycogen synthase kinase 3 alpha antibody
    • Glycogen synthase kinase 3 beta antibody
    • GSK3 alpha antibody
    • GSK3 beta antibody
    • GSK3B antibody
    see all

Anti-GSK3 (alpha + beta) antibody 图像

  • ab15314 (1/100) staining GSK3 in formalin fixed, paraffin-embbeded human brain tissue sections. Antigen retrieval was performed by boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
  • ab15314 staining GSK3 (alpha + beta) in Vero cells infected with HSV1 by Immunocytochemistry.

    Cells were infected with HSV1 SC16 at an MOI of 1 for 16 hours, fixed with formaldehyde and blocked by TBS containing 0.025% Triton X-100. Samples were incubated with primary antibody (1/100 in diluent) for 10 minutes.

Anti-GSK3 (alpha + beta) antibody (ab15314)参考文献

This product has been referenced in:
  • Wozniak MA  et al. Antivirals reduce the formation of key Alzheimer's disease molecules in cell cultures acutely infected with herpes simplex virus type 1. PLoS One 6:e25152 (2011). ICC ; African Green Monkey . Read more (PubMed: 22003387) »
  • Wozniak MA  et al. Alzheimer's disease-specific tau phosphorylation is induced by herpes simplex virus type 1. J Alzheimers Dis 16:341-50 (2009). ICC/IF ; Human . Read more (PubMed: 19221424) »

See all 2 Publications for this product

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