Lane 1: GSK3A knockout HAP1 whole cell lysate (20 µg) Lane 2: Wild type HAP1 whole cell lysate (20 µg) Lane 3: HeLa whole cell lysate (20 µg) Lane 4: HepG2 whole cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab78664 observed at 52 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab78664 was shown to recognize GSK3A when GSK3A knockout samples were used, along with additional cross-reactive bands. Wild-type and GSK3A knockout samples were subjected to SDS-PAGE. Followed by incubation with ab78664 and ab8245 (Mouse anti-GAPDH loading control) overnight at 4°C at a 1/250 and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
Western blot - GSK3 alpha antibody (ab78664)
All lanes : Anti-GSK3 alpha antibody (ab78664) at 1/250 dilution
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution