概述

  • 产品名称Anti-GSE1抗体
  • 描述
    兔多克隆抗体to GSE1
  • 经测试应用适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within the N terminal amino acids 71-120 (EEPRGSSLSS ESSPVSSPAT NHSSPASTPK RVPMGPIIVP PGGHSVPSTP) of Human GSE1 (NP_055430)

  • 阳性对照
    • 721_B cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab83553 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay 1/312500.

WB Use a concentration of 1 µg/ml. Detects a band of approximately 144 kDa (predicted molecular weight: 136 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

Anti-GSE1 antibody 图像

  • Anti-GSE1 antibody (ab83553) at 1 µg/ml + 721_B cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 136 kDa
    Observed band size : 144 kDa (why is the actual band size different from the predicted?)

Anti-GSE1 antibody (ab83553)参考文献

ab83553 has not yet been referenced specifically in any publications.

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