概述

  • 产品名称
  • 描述
    兔多克隆抗体to GSC2
  • 特异性
    ab65032 detects endogenous levels of total GSC2 protein.
  • 经测试应用
    适用于: ELISA, WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide derived from an internal sequence of human GSC2.

  • 阳性对照
    • 293, HepG2 and COLO205 cell extracts.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    ab65032 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab65032 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/20000.
WB 1/500 - 1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 22 kDa).
IHC-P 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

靶标

  • 相关性
    GSC2, also known as Goosecoidlike or GSCL, is a homeodomain-containing gene that resides in the critical region for VCFS/DGS on 22q11. Velocardiofacial syndrome (VCFS) is a developmental disorder characterized by conotruncal heart defects, craniofacial anomalies, and learning disabilities and is phenotypically related to DiGeorge syndrome (DGS), both of which are associated with hemizygous 22q11 deletions.
  • 细胞定位
    Nuclear
  • 数据库链接
  • 别名
    • GSC 2 antibody
    • GSCL antibody
    • Homeobox protein goosecoid 2 antibody
    • Homeobox protein goosecoid like antibody

Anti-GSC2 antibody 图像

  • All lanes : Anti-GSC2 antibody (ab65032) at 1/500 dilution

    Lane 1 : 293 cell extract
    Lane 2 : HepG2 cell extract
    Lane 3 : COLO205 cell extract
    Lane 4 : COLO205 cell extract with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 22 kDa
    Observed band size : 45 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 28 kDa,34 kDa. We are unsure as to the identity of these extra bands.
  • Immunostaining of mouse E9.5 embryo tissue sections. After using a biotinylated secondary, ABC and then DAB was used for color development. Please see abreview for protocol details.

    See Abreview

Anti-GSC2 antibody (ab65032)参考文献

ab65032 has not yet been referenced specifically in any publications.

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Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse ES Cells)
Loading amount
50 µg
Specification
Mouse ES Cells
Treatment
Differentiating Conditions (Minus LIF Medium)
Gel Running Conditions
Reduced Denaturing (15%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Mr. Utkarsh Kapoor

Verified customer

提交于 Dec 31 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (E9.5 embryo)
Specification
E9.5 embryo
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric Acid
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Username

Dr. Jesse Mager

Verified customer

提交于 Feb 13 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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