The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 22 kDa).
1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
GSC2, also known as Goosecoidlike or GSCL, is a homeodomain-containing gene that resides in the critical region for VCFS/DGS on 22q11. Velocardiofacial syndrome (VCFS) is a developmental disorder characterized by conotruncal heart defects, craniofacial anomalies, and learning disabilities and is phenotypically related to DiGeorge syndrome (DGS), both of which are associated with hemizygous 22q11 deletions.