重组
RabMAb

Anti-GSC抗体[EPR4847] (ab109024)

概述

  • 产品名称
    Anti-GSC抗体[EPR4847]
    参阅全部 GSC 一抗
  • 描述
    兔单克隆抗体[EPR4847] to GSC
  • 特异性
    Negative WB result with mouse brain, heart, kidney and spleen tissue lysates, but there is a feedback this antibody detected mouse GSC with NMuMG (mouse mammary gland, normal epithelial) cell lysate. Other samples has not been tested.
  • 经测试应用
    适用于: WB, IPmore details
    不适用于: Flow Cyt,ICC or IHC-P
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to residues in Human GSC.

  • 阳性对照
    • Human brain, HT29, MCF7, and HepG2 lysates.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab109024 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).
IP 1/10 - 1/100.
  • 应用说明
    Is unsuitable for Flow Cyt,ICC or IHC-P.
  • 靶标

    • 功能
      Regulates chordin (CHRD). May play a role in spatial programing within discrete embryonic fields or lineage compartments during organogenesis. In concert with NKX3-2, plays a role in defining the structural components of the middle ear; required for the development of the entire tympanic ring.
    • 序列相似性
      Belongs to the paired homeobox family. Bicoid subfamily.
      Contains 1 homeobox DNA-binding domain.
    • 细胞定位
      Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Goosecoid antibody
      • goosecoid homeobox antibody
      • GSC antibody
      • GSC_HUMAN antibody
      • Homeobox protein goosecoid antibody
      see all

    Anti-GSC antibody [EPR4847] 图像

    • All lanes : Anti-GSC antibody [EPR4847] (ab109024) at 1/1000 dilution

      Lane 1 : Human brain lysate
      Lane 2 : HT29 lysate
      Lane 3 : MCF7 lysate
      Lane 4 : HepG2 lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 28 kDa

    Anti-GSC antibody [EPR4847] (ab109024)参考文献

    ab109024 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Mouse embryonic stem cells)
    Gel Running Conditions
    Reduced Denaturing (Bis-Tris from Invitrogen 4-12%)
    Loading amount
    18 µg
    Specification
    Mouse embryonic stem cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Miss. Maud De Dieuleveult

    Verified customer

    提交于 Jul 04 2017

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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