重组
RabMAb

Anti-GSC抗体[EPR4847] (ab109024)

概述

  • 产品名称
    Anti-GSC抗体[EPR4847]
    参阅全部 GSC 一抗
  • 描述
    兔单克隆抗体[EPR4847] to GSC
  • 特异性
    Negative WB result with mouse brain, heart, kidney and spleen tissue lysates, but there is a feedback this antibody detected mouse GSC with NMuMG (mouse mammary gland, normal epithelial) cell lysate. Other samples has not been tested.
  • 经测试应用
    适用于: WB, IPmore details
    不适用于: Flow Cyt,ICC or IHC-P
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to residues in Human GSC.

  • 阳性对照
    • Human brain, HT29, MCF7, and HepG2 lysates.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab109024 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).
IP 1/10 - 1/100.
  • 应用说明
    Is unsuitable for Flow Cyt,ICC or IHC-P.
  • 靶标

    • 功能
      Regulates chordin (CHRD). May play a role in spatial programing within discrete embryonic fields or lineage compartments during organogenesis. In concert with NKX3-2, plays a role in defining the structural components of the middle ear; required for the development of the entire tympanic ring.
    • 序列相似性
      Belongs to the paired homeobox family. Bicoid subfamily.
      Contains 1 homeobox DNA-binding domain.
    • 细胞定位
      Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Goosecoid antibody
      • goosecoid homeobox antibody
      • GSC antibody
      • GSC_HUMAN antibody
      • Homeobox protein goosecoid antibody
      see all

    Anti-GSC antibody [EPR4847] 图像

    • All lanes : Anti-GSC antibody [EPR4847] (ab109024) at 1/1000 dilution

      Lane 1 : Human brain lysate
      Lane 2 : HT29 lysate
      Lane 3 : MCF7 lysate
      Lane 4 : HepG2 lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 28 kDa

    Anti-GSC antibody [EPR4847] (ab109024)参考文献

    ab109024 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Mouse embryonic stem cells)
    Gel Running Conditions
    Reduced Denaturing (Bis-Tris from Invitrogen 4-12%)
    Loading amount
    18 µg
    Specification
    Mouse embryonic stem cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Miss. Maud De Dieuleveult

    Verified customer

    提交于 Jul 04 2017

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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