概述

  • 产品名称
  • 描述
    兔多克隆抗体to GRPEL2
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • 免疫原

    A synthetic peptide corresponding to a region within C terminal sequence 176-225 (

    HEHELICHVPAGVGVQPGTVALVRQDGYKLHGRTIRLARVEVAVESQRRL

    ) of Human GRPEL2, NP_689620

  • 阳性对照
    • Human fetal heart lysate

性能

应用

Our Abpromise guarantee covers the use of ab85428 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 25 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

图片

  • Anti-GRPEL2 antibody (ab85428) at 1 µg/ml (in 5% skim milk / PBS buffer) + fetal heart lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 25 kDa
    Observed band size: 28 kDa (why is the actual band size different from the predicted?)



    Gel concentration 12%

实验方案

文献

ab85428 has not yet been referenced specifically in any publications.

客户评价及客户问答

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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