Anti-GRP94抗体(ab53075)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to GRP94
  • 特异性
    GRP94 antibody detects endogenous levels of total GRP94 protein.
  • 经测试应用
    适用于: WB, IHC-P, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide (Human)

  • 阳性对照
    • human breast carcinoma tissue; SKOV3 cell extract

性能

应用

Our Abpromise guarantee covers the use of ab53075 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/300 - 1/1000. Detects a band of approximately 92 kDa (predicted molecular weight: 92 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/5000.

靶标

  • 功能
    Molecular chaperone that functions in the processing and transport of secreted proteins. Functions in endoplasmic reticulum associated degradation (ERAD). Has ATPase activity.
  • 序列相似性
    Belongs to the heat shock protein 90 family.
  • 细胞定位
    Endoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 94 kDa glucose regulated protein antibody
    • 94 kDa glucose-regulated protein antibody
    • ECGP antibody
    • Endoplasmin antibody
    • Endothelial cell (HBMEC) glycoprotein antibody
    • ENPL_HUMAN antibody
    • Glucose regulated protein 94kDa antibody
    • gp96 antibody
    • gp96 homolog antibody
    • GRP 94 antibody
    • GRP-94 antibody
    • Heat shock protein 90 kDa beta member 1 antibody
    • heat shock protein 90kDa beta (Grp94), member 1 antibody
    • Heat shock protein, 90 kDa, beta, 1 antibody
    • HSP90B1 antibody
    • Stress inducible tumor rejection antigen GP96 antibody
    • TRA1 antibody
    • tumor rejection antigen (gp96) 1 antibody
    • Tumor rejection antigen 1 antibody
    • Tumor rejection antigen gp96 antibody
    • Tumor rejection antigen-1 (gp96) antibody
    see all

图片

  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue
    using ab53075 at 1/50 dilution, with and without immunizing peptide.
  • All lanes : Anti-GRP94 antibody (ab53075) at 1/300 dilution

    Lane 1 : SKOV3 cell extract with no immunizing peptide
    Lane 2 : SKOV3 cell extract with immunizing peptide


    Predicted band size : 92 kDa
    Observed band size : 92 kDa


  • Performed under reducing conditions.

    Predicted band size : 92 kDa
    Observed band size : 92 kDa

    This image is courtesy of an anonymous Abreview

    See Abreview

文献

ab53075 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Tissue lysate - whole (Prostate- BPH)
Loading amount
10 µg
Specification
Prostate- BPH
Treatment
10uM SF for 24 hours
Gel Running Conditions
Reduced Denaturing (10% gel, MOPS buffer)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Mar 19 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Prostate)
Specification
Prostate
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric Acid Buffer
Permeabilization
No
Blocking step
10% horse serum + 1% BSA in TBST as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Mar 11 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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