概述

  • 产品名称Anti-GRP94抗体
    参阅全部 GRP94 一抗
  • 描述
    兔多克隆抗体to GRP94
  • 经测试应用适用于: ICC, ICC/IF, WBmore details
  • 种属反应性
    与反应: Hamster, Human, Monkey
    不与反应: Mouse, Rat
  • 免疫原

    Synthetic peptide (Human) (Internal sequence).

性能

应用

Our Abpromise guarantee covers the use of ab18055 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC
ICC/IF
WB
  • 应用说明
    ICC: 1/100 - 1/400.
    IF: 1/100 - 1/400.
    WB: 1/500 - 1/1000. Detects a band of approximately 100 kDa (predicted molecular weight: 94 kDa).

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    Works in ICC/IF with methanol-fixed and 4% paraformaldehyde fixed cells.
  • 靶标

    • 功能Molecular chaperone that functions in the processing and transport of secreted proteins. Functions in endoplasmic reticulum associated degradation (ERAD). Has ATPase activity.
    • 序列相似性Belongs to the heat shock protein 90 family.
    • 细胞定位Endoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 94 kDa glucose regulated protein antibody
      • 94 kDa glucose-regulated protein antibody
      • ECGP antibody
      • Endoplasmin antibody
      • Endothelial cell (HBMEC) glycoprotein antibody
      • ENPL_HUMAN antibody
      • Glucose regulated protein 94kDa antibody
      • gp96 antibody
      • gp96 homolog antibody
      • GRP 94 antibody
      • GRP-94 antibody
      • Heat shock protein 90 kDa beta member 1 antibody
      • heat shock protein 90kDa beta (Grp94), member 1 antibody
      • Heat shock protein, 90 kDa, beta, 1 antibody
      • HSP90B1 antibody
      • Stress inducible tumor rejection antigen GP96 antibody
      • TRA1 antibody
      • tumor rejection antigen (gp96) 1 antibody
      • Tumor rejection antigen 1 antibody
      • Tumor rejection antigen gp96 antibody
      • Tumor rejection antigen-1 (gp96) antibody
      see all

    Anti-GRP94 antibody 图像

    • Methanol-fixed HEK293 cells stained at 1/200 with ab18055 followed by FITC conjugated secondary.



    • Predicted band size : 94 kDa


      Western blot of soluble fraction of monkey brain stained at 1/1000 with ab18055, visualised with HRP-conjugated secondary and ECL.

    Anti-GRP94 antibody (ab18055)参考文献

    This product has been referenced in:
    • Weston CJ  et al. Cellular localization and trafficking of vascular adhesion protein-1 as revealed by an N-terminal GFP fusion protein. J Neural Transm 120:951-61 (2013). ICC/IF ; Human . Read more (PubMed: 23474851) »
    • Mulya A  et al. Apolipoprotein M expression increases the size of nascent pre beta HDL formed by ATP binding cassette transporter A1. J Lipid Res 51:514-24 (2010). ICC/IF ; Human . Read more (PubMed: 19767535) »

    See all 3 Publications for this product

    Product Wall

    Wir haben leider aktuell keinen validierten anti-Housekeeping Protein Antikörper der nur humane Proteine erkennt. Wir wissen, dass der ab3369 Click here (or use the following: http://www.abcam.com/index.html?datasheet=3369) gegen MRP1 nicht mit der Mau...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (huh-7)
    Loading amount 15000 cells
    Specification huh-7
    Gel Running Conditions Reduced Denaturing (12%)
    Blocking step Milk as blocking agent for 20 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Username

    Abcam user community

    Verified customer

    提交于 Nov 13 2008

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Hamster Cell lysate - whole cell (Chinese Hamster Ovary cell line)
    Loading amount 50 µg
    Specification Chinese Hamster Ovary cell line
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4%
    Username

    Abcam user community

    Verified customer

    提交于 Aug 11 2006

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"