Anti-GRP94抗体(ab13509)
Key features and details
- Rabbit polyclonal to GRP94
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-GRP94抗体
参阅全部 GRP94 一抗 -
描述
兔多克隆抗体to GRP94 -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide corresponding to Mouse GRP94 aa 787-802 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
Sequence:EEEEETEKESTEKDEL
Database link: P08113 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab13509于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (1) |
Use at an assay dependent concentration. Predicted molecular weight: 94 kDa. 2µg/mL of ab13509 detects of Grp94 in 20µg of Rat Brain Tissue Extract using colorimetric analysis. 1/2000 for ECL detection.
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ICC/IF |
Use a concentration of 1 µg/ml.
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说明 |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 94 kDa. 2µg/mL of ab13509 detects of Grp94 in 20µg of Rat Brain Tissue Extract using colorimetric analysis. 1/2000 for ECL detection. |
ICC/IF
Use a concentration of 1 µg/ml. |
靶标
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功能
Molecular chaperone that functions in the processing and transport of secreted proteins. Functions in endoplasmic reticulum associated degradation (ERAD). Has ATPase activity. -
序列相似性
Belongs to the heat shock protein 90 family. -
细胞定位
Endoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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数据库链接
- Entrez Gene: 7184 Human
- Entrez Gene: 22027 Mouse
- Entrez Gene: 362862 Rat
- Omim: 191175 Human
- SwissProt: P14625 Human
- SwissProt: P08113 Mouse
- SwissProt: Q66HD0 Rat
- Unigene: 192374 Human
see all -
别名
- 94 kDa glucose regulated protein antibody
- 94 kDa glucose-regulated protein antibody
- ECGP antibody
see all
图片
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All lanes : Anti-GRP94 antibody (ab13509) at 1/1000 dilution
Lane 1 : Molecular weight ladder
Lane 2 : Lysates prepared from rat brain
Lane 3 : Lysates prepared from mouse brain
Lane 4 : Lysates prepared from rat liver microsomes
Lane 5 : Lysates prepared from mouse liver microsomes
Lane 6 : Lysates prepared from human liver microsomes
Predicted band size: 94 kDa -
ICC/IF image of ab13509 stained rat PC12 cells. The cells were 4% PFA and 100% methanol fixed (10 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab13509, 1 µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue).
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (19)
ab13509 被引用在 19 文献中.
- Meng Q et al. Human bone marrow mesenchymal stem cell-derived extracellular vesicles impede the progression of cervical cancer via the miR-144-3p/CEP55 pathway. J Cell Mol Med 25:1867-1883 (2021). PubMed: 33417281
- Wang X et al. Identification of small extracellular vesicle subtypes in follicular fluid: Insights into the function and miRNA profiles. J Cell Physiol 236:5633-5645 (2021). PubMed: 33576507
- Miraglia F et al. Alpha-Synuclein FRET Biosensors Reveal Early Alpha-Synuclein Aggregation in the Endoplasmic Reticulum. Life (Basel) 10:N/A (2020). PubMed: 32796544
- Yao X et al. Endoplasmic reticulum stress-induced exosomal miR-27a-3p promotes immune escape in breast cancer via regulating PD-L1 expression in macrophages. J Cell Mol Med 24:9560-9573 (2020). PubMed: 32672418
- Ou H et al. Extracellular vesicles derived from microRNA-150-5p-overexpressing mesenchymal stem cells protect rat hearts against ischemia/reperfusion. Aging (Albany NY) 12:12669-12683 (2020). PubMed: 32657760