概述

  • 产品名称Anti-GRP94抗体[9G10]
    参阅全部 GRP94 一抗
  • 描述
    大鼠单克隆抗体[9G10] to GRP94
  • 经测试应用适用于: WB, ICC/IF, ICC, IHC-P, IPmore details
  • 种属反应性
    与反应: Mouse, Rat, Chicken, Hamster, Cow, Human, Pig
    预测可用于: Sheep, Rabbit, Dog, Xenopus laevis, Drosophila melanogaster, Non Human Primates
  • 免疫原

    Full length protein corresponding to Chicken GRP94. Chick oviduct GRP94.

性能

应用

Our Abpromise guarantee covers the use of ab2791 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/5000.
ICC/IF 1/250.
ICC 1/250.
IHC-P 1/200.
IP Use at an assay dependent concentration.

靶标

Anti-GRP94 antibody [9G10] 图像

  • Immunocytochemistry/Immunofluorescence analysis of GRP94 shows staining in C6 cells. GRP94 (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with ab2791 (1:100) overnight at 4ºC, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.

  • Immunocytochemistry/Immunofluorescence analysis of GRP94 shows staining in HeLa cells. GRP94 (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with ab2791 (1:100) overnight at 4ºC, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.

  • Immunocytochemistry/Immunofluorescence analysis of GRP94 shows staining in NIH-3T3 cells. GRP94 (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with ab2791 (1:20) overnight at 4ºC, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.

  • ab2791 labelling GRP94 in Human colon adenocarcinoma tissue sections by Immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). To expose target proteins, heat-induced epitope retrieval was performed using 10mM sodium citrate (pH 6.0) buffer for 20 minutes at 95ºC. Following antigen retrieval, tissues were blocked in 3% BSA in PBST for 30 minutes at room temperature. Tissue sections were incubated with the primary antibody (1:100) for 1 hour (right panel). Negative control - left panel. Endogenous peroxidase activity quenched with Peroxidase Suppressor for 30 minutes at room temperature. A HRP-conjugated Goat anti-rat IgG was used as the secondary antibody (1:500), followed by colorimetric detection using Metal Enhanced DAB Substrate Kit. Tissues were counterstained with hematoxylin and prepped for mouting. Images were taken at 40X magnification.

  • ab2791 labelling Grp94 in U2OS cells bu immunocytochemistry. Cells fixed with 4% paraformaldehyde were permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature and blocked with 2% BSA in PBST for 30 minutes at room temperature. Cells were treated with Peroxidase Suppressor, and incubated with the primary antibody (1:100) for 1 hour at room temperature. A HRP-conjugated Goat anti-rat IgG (H+L) was used as the secondary antibody (1:1000 for 30 minutes at room temperature). Chromogenic detection was performed using Metal Enhanced DAB Substrate Kit. Images were taken on a Zeiss Axio Observer microscope at 20X magnification (x1.6 Optovar ~ 32X).

  • Immunohistochemistry was performed on normal biopsies of deparaffinized Mouse liver tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing Glucose Regulated Protein 94 ab2791 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

  • Immunohistochemistry was performed on normal biopsies of deparaffinized Mouse lymph node. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing Glucose Regulated Protein 94 ab2791 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

  • Immunohistochemistry was performed on normal biopsies of deparaffinized Mouse breast tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing Glucose Regulated Protein 94 ab2791 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

Anti-GRP94 antibody [9G10] (ab2791)参考文献

This product has been referenced in:
  • Verma S  et al. Inhibition of the TRAIL death receptor by CMV reveals its importance in NK cell-mediated antiviral defense. PLoS Pathog 10:e1004268 (2014). ICC/IF ; Mouse . Read more (PubMed: 25122141) »
  • Yu S  et al. Global ablation of the mouse Rab11a gene impairs early embryogenesis and matrix metalloproteinase secretion. J Biol Chem 289:32030-43 (2014). Read more (PubMed: 25271168) »

See all 6 Publications for this product

Product Wall

Regarding ab2791, I have the following specific reference which cites the use of this antibody in IF. Repro. Bio and Endo: 1:17, 2003 http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=12646049 For IF, the author uses this...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"