概述

  • 产品名称Anti-GRP94抗体[2H3]
    参阅全部 GRP94 一抗
  • 描述
    小鼠单克隆抗体[2H3] to GRP94
  • 经测试应用适用于: WB, ELISA, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 676-803 of Human GRP94, purified from E. coli.

  • 阳性对照
    • Human embryonic stem cell lysate. Human embryonic stem cells.

性能

应用

Our Abpromise guarantee covers the use of ab63469 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/100 - 1/500. Detects a band of approximately 98 kDa (predicted molecular weight: 92 kDa).
ELISA 1/100.
ICC/IF 1/100 - 1/200.

靶标

  • 功能Molecular chaperone that functions in the processing and transport of secreted proteins. Functions in endoplasmic reticulum associated degradation (ERAD). Has ATPase activity.
  • 序列相似性Belongs to the heat shock protein 90 family.
  • 细胞定位Endoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 94 kDa glucose regulated protein antibody
    • 94 kDa glucose-regulated protein antibody
    • ECGP antibody
    • Endoplasmin antibody
    • Endothelial cell (HBMEC) glycoprotein antibody
    • ENPL_HUMAN antibody
    • Glucose regulated protein 94kDa antibody
    • gp96 antibody
    • gp96 homolog antibody
    • GRP 94 antibody
    • GRP-94 antibody
    • Heat shock protein 90 kDa beta member 1 antibody
    • heat shock protein 90kDa beta (Grp94), member 1 antibody
    • Heat shock protein, 90 kDa, beta, 1 antibody
    • HSP90B1 antibody
    • Stress inducible tumor rejection antigen GP96 antibody
    • TRA1 antibody
    • tumor rejection antigen (gp96) 1 antibody
    • Tumor rejection antigen 1 antibody
    • Tumor rejection antigen gp96 antibody
    • Tumor rejection antigen-1 (gp96) antibody
    see all

Anti-GRP94 antibody [2H3] 图像

  • Anti-GRP94 antibody [2H3] (ab63469) at 1/500 dilution + Human embryonic stem cell lysate at 20 µg

    Secondary
    Goat anti-mouse secondary antibody conjugated to HRP
    Developed using the ECL technique

    Predicted band size : 92 kDa
    Observed band size : 98 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 210 kDa,250 kDa,60 kDa,90 kDa. We are unsure as to the identity of these extra bands.
  • Immunofluorescence staining of human embryonic stem cell colony with ab63469 (1:100).

Anti-GRP94 antibody [2H3] (ab63469)参考文献

This product has been referenced in:
  • Muinelo-Romay L  et al. Identification of a(1,6)fucosylated proteins differentially expressed in human colorectal cancer. BMC Cancer 11:508 (2011). WB ; Human . Read more (PubMed: 22152070) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - whole (Normal colorectal tissue)
Loading amount 30 µg
Specification Normal colorectal tissue
Gel Running Conditions Reduced Denaturing (10)
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Dr. Lay Harn Gam

Verified customer

提交于 May 24 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"