概述

  • 产品名称Anti-GRP78 BiP抗体
    参阅全部 GRP78 BiP 一抗
  • 描述
    兔多克隆抗体to GRP78 BiP
  • 经测试应用适用于: ICC/IF, WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Dog, Human
  • 免疫原

    Recombinant full length Human GRP78 BiP (His tagged at the N terminus).

  • 阳性对照
    • WB: Recombinant human GRP78 BiP and SKBR3, MDCK, and MEF cell lysates. ICC/IF: HeLa cell line. IHC-P: Human breast fibroadenoma tissue.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: 50% Glycerol, PBS
  • Concentration information loading...
  • 纯度Protein A purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab109659 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB 1/1000 - 1/2000. Detects a band of approximately 78 kDa (predicted molecular weight: 72 kDa).
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Probably plays a role in facilitating the assembly of multimeric protein complexes inside the endoplasmic reticulum. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10, probably to facilitate the release of DNAJC10 from its substrate.
  • 疾病相关Autoantigen in rheumatoid arthritis.
  • 序列相似性Belongs to the heat shock protein 70 family.
  • 细胞定位Endoplasmic reticulum lumen. Melanosome. Cytoplasm. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 78 kDa glucose regulated protein antibody
    • 78 kDa glucose-regulated protein antibody
    • AL022860 antibody
    • AU019543 antibody
    • BIP antibody
    • D2Wsu141e antibody
    • D2Wsu17e antibody
    • Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78 antibody
    • Endoplasmic reticulum lumenal Ca2+ binding protein grp78 antibody
    • Epididymis secretory sperm binding protein Li 89n antibody
    • FLJ26106 antibody
    • Glucose Regulated Protein 78kDa antibody
    • GRP 78 antibody
    • GRP-78 antibody
    • GRP78 antibody
    • GRP78_HUMAN antibody
    • Heat shock 70 kDa protein 5 antibody
    • Heat Shock 70kDa Protein 5 antibody
    • Heat shock protein family A (Hsp70) member 5 antibody
    • HEL S 89n antibody
    • Hsce70 antibody
    • HSPA 5 antibody
    • HSPA5 antibody
    • Immunoglobulin Heavy Chain Binding Protein antibody
    • Immunoglobulin heavy chain-binding protein antibody
    • mBiP antibody
    • MIF2 antibody
    • Sez7 antibody
    see all

Anti-GRP78 BiP antibody 图像

  • IHC image of GRP78 BiP staining in Human breast fibroadenoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab109659, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • ICC/IF image of ab109659 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab109659, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 Goat anti-Rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-GRP78 BiP antibody (ab109659) at 1 µg/ml

    Lane 1 : Recombinant Human GRP78 BiP at 0.1 µg
    Lane 2 : SKBR3 lysate (Human) at 7.5 µg
    Lane 3 : MDCK lysate (Dog) at 7.5 µg
    Lane 4 : MEF lysate (Mouse) at 7.5 µg


    Predicted band size : 72 kDa
    Observed band size : 78 kDa (why is the actual band size different from the predicted?)

Anti-GRP78 BiP antibody (ab109659)参考文献

ab109659 has not yet been referenced specifically in any publications.

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