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The details of the immunogen for this antibody are not available.
Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause.
Our Abpromise guarantee covers the use of ab25192 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent dilution. Immunoglobulin assembly and secretion.|
|IHC-P||Use a concentration of 10 µg/ml.|
|WB||Use at an assay dependent dilution. Predicted molecular weight: 72 kDa.|
|ELISA||Use at an assay dependent dilution.|
IHC image of GRP78 BiP staining in XHuman breast fibroadenoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab25192, 10µg/ml, for 15 mins at room temperature. A Goat anti-Rat biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Total cell lysates from Ag8.653 cells were resolved by electrophoresis, transferred to PVDF membrane, and probed with Anti-GRP78 BiP antibody [76-E6] ab25192 (2 µg/mL). Proteins were visualized using Goat Anti-Rat Ig, Mouse ads-HRP secondary antibody and chemiluminescent detection.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"