概述

  • 产品名称Anti-GRP78 BiP抗体[76-E6]
    参阅全部 GRP78 BiP 一抗
  • 描述
    大鼠单克隆抗体[76-E6] to GRP78 BiP
  • 特异性This antibody has been shown to be useful in studies of immunoglobulin assembly and secretion.
  • 经测试应用适用于: Functional Studies, IHC-P, WB, ELISAmore details
  • 种属反应性
    与反应: Mouse, Hamster, Human
  • 免疫原

    The details of the immunogen for this antibody are not available.

  • 表位This antibody recognizes a conserved epitope localized within the region of amino acids 497 to 581 of BiP.
  • 阳性对照
    • This antibody gave a positive result in IHC in the following FFPE tissue: Human breast fibroadenoma.
  • 常规说明


性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液100 mM borate buffered saline, pH 8.0.
  • Concentration information loading...
  • 纯度IgG fraction
  • 克隆单克隆
  • 克隆编号76-E6
  • 同种型IgG1
  • 轻链类型kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab25192 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Functional Studies Use at an assay dependent dilution. Immunoglobulin assembly and secretion.
IHC-P Use a concentration of 10 µg/ml.
WB Use at an assay dependent dilution. Predicted molecular weight: 72 kDa.
ELISA Use at an assay dependent dilution.

靶标

  • 功能Probably plays a role in facilitating the assembly of multimeric protein complexes inside the endoplasmic reticulum. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10, probably to facilitate the release of DNAJC10 from its substrate.
  • 疾病相关Autoantigen in rheumatoid arthritis.
  • 序列相似性Belongs to the heat shock protein 70 family.
  • 细胞定位Endoplasmic reticulum lumen. Melanosome. Cytoplasm. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 78 kDa glucose regulated protein antibody
    • 78 kDa glucose-regulated protein antibody
    • AL022860 antibody
    • AU019543 antibody
    • BIP antibody
    • D2Wsu141e antibody
    • D2Wsu17e antibody
    • Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78 antibody
    • Endoplasmic reticulum lumenal Ca2+ binding protein grp78 antibody
    • Epididymis secretory sperm binding protein Li 89n antibody
    • FLJ26106 antibody
    • Glucose Regulated Protein 78kDa antibody
    • GRP 78 antibody
    • GRP-78 antibody
    • GRP78 antibody
    • GRP78_HUMAN antibody
    • Heat shock 70 kDa protein 5 antibody
    • Heat Shock 70kDa Protein 5 antibody
    • Heat shock protein family A (Hsp70) member 5 antibody
    • HEL S 89n antibody
    • Hsce70 antibody
    • HSPA 5 antibody
    • HSPA5 antibody
    • Immunoglobulin Heavy Chain Binding Protein antibody
    • Immunoglobulin heavy chain-binding protein antibody
    • mBiP antibody
    • MIF2 antibody
    • Sez7 antibody
    see all

Anti-GRP78 BiP antibody [76-E6] 图像

  • IHC image of GRP78 BiP staining in XHuman breast fibroadenoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab25192, 10µg/ml, for 15 mins at room temperature. A Goat anti-Rat biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-GRP78 BiP antibody [76-E6] (ab25192)参考文献

This product has been referenced in:
  • Fausther-Bovendo H  et al. HIV gp41 engages gC1qR on CD4+ T cells to induce the expression of an NK ligand through the PIP3/H2O2 pathway. PLoS Pathog 6:e1000975 (2010). ICC/IF ; Human . Read more (PubMed: 20617170) »

See 1 Publication for this product

Product Wall

Application ELISA
Sample Human Recombinant protein (Blood Plasma)
Specification Blood Plasma
Type Sandwich (Capture)
Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Abcam user community

Verified customer

提交于 Aug 16 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: sodium citrate
Sample Mouse Tissue sections (brain hemisphere coronal sections)
Specification brain hemisphere coronal sections
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 2 · Temperature: 25°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Jul 16 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"