Anti-Grp75抗体(ab92808)

概述

  • 产品名称Anti-Grp75抗体
    参阅全部 Grp75 一抗
  • 描述
    兔多克隆抗体to Grp75
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Cow, Chinese Hamster, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human Grp75.

  • 阳性对照
    • This antibody gave a positive signal in the following lysates: Human Brain Tissue; Mouse Brain Tissue; Rat Brain Tissue; Human kidney tissue; HeLa Whole Cell; HepG2 Whole Cell Lysate; HEK293 Whole Cell Lysate.

性能

应用

Our Abpromise guarantee covers the use of ab92808 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa).

靶标

  • 功能Implicated in the control of cell proliferation and cellular aging. May also act as a chaperone.
  • 序列相似性Belongs to the heat shock protein 70 family.
  • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位Mitochondrion.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 75 kDa glucose regulated protein antibody
    • 75 kDa glucose-regulated protein antibody
    • CSA antibody
    • Glucose Regulated Protein antibody
    • Grp 75 antibody
    • GRP-75 antibody
    • GRP75 antibody
    • GRP75_HUMAN antibody
    • Heat shock 70 kDa protein 9 antibody
    • Heat shock 70kD protein 9 antibody
    • heat shock 70kDa protein 9 antibody
    • Heat shock 70kDa protein 9B antibody
    • Heat shock protein 74 kDa A antibody
    • Heat shock protein A antibody
    • Heat shock protein cognate 74 antibody
    • Hsc74 antibody
    • Hsp74 antibody
    • Hsp74a antibody
    • HSPA9 antibody
    • Hspa9a antibody
    • HSPA9B antibody
    • MGC4500 antibody
    • mitochondrial antibody
    • Mortalin 2 antibody
    • Mortalin antibody
    • Mortalin perinuclear antibody
    • Mortalin2 antibody
    • MOT 2 antibody
    • MOT antibody
    • MOT2 antibody
    • Mthsp70 antibody
    • p66 mortalin antibody
    • P66 MOT antibody
    • PBP74 antibody
    • Peptide binding protein 74 antibody
    • Peptide-binding protein 74 antibody
    • Stress 70 protein mitochondrial antibody
    • Stress 70 protein mitochondrial precursor antibody
    • Stress-70 protein antibody
    see all

Anti-Grp75 antibody 图像

  • All lanes : Anti-Grp75 antibody (ab92808) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Brain (Mouse) Tissue Lysate
    Lane 3 : Brain (Rat) Tissue Lysate
    Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 6 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 7 : Human kidney tissue lysate - total protein (ab30203)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 74 kDa
    Observed band size : 74 kDa


    Exposure time : 30 seconds
  • ICC/IF image of ab92808 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab92808, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) MCF7 cells at 5µg/ml.

Anti-Grp75 antibody (ab92808)参考文献

ab92808 has not yet been referenced specifically in any publications.

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Thank you for submitting an Abreview of ab92808. As you may have noticed, your review has now been published on our website.
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Abcam has not validated the combination of species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Cell lysate - whole cell (LNCaP)
Total protein in input 120 µg
Specification LNCaP
Immuno-precipitation step Protein A/G
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提交于 May 16 2012

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