Anti-Grp75/MOT抗体(ab227215)
Key features and details
- Rabbit polyclonal to Grp75/MOT
- Suitable for: IP, IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Grp75/MOT抗体
参阅全部 Grp75/MOT 一抗 -
描述
兔多克隆抗体to Grp75/MOT -
宿主
Rabbit -
经测试应用
适用于: IP, IHC-P, WB, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Human
预测可用于: Chicken, Cow, Xenopus laevis -
免疫原
Recombinant fragment within Human Grp75/MOT (internal sequence). The exact sequence is proprietary.
Database link: P38646 -
阳性对照
- WB: HEK-293T, A431, HeLa, HepG2 and SCC-15 whole cell extract. IHC-P: Mouse duodenum tissue; rat duodenum and brain tissues. IP: HepG2 whole cell extract.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab227215于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IP |
1/100 - 1/500.
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IHC-P |
1/100 - 1/1000.
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WB |
1/500 - 1/3000. Predicted molecular weight: 74 kDa.
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ICC/IF |
1/500.
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说明 |
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IP
1/100 - 1/500. |
IHC-P
1/100 - 1/1000. |
WB
1/500 - 1/3000. Predicted molecular weight: 74 kDa. |
ICC/IF
1/500. |
靶标
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功能
Implicated in the control of cell proliferation and cellular aging. May also act as a chaperone. -
序列相似性
Belongs to the heat shock protein 70 family. -
翻译后修饰
Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Mitochondrion. - Information by UniProt
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数据库链接
- Entrez Gene: 416183 Chicken
- Entrez Gene: 517535 Cow
- Entrez Gene: 3313 Human
- Entrez Gene: 15526 Mouse
- Entrez Gene: 291671 Rat
- Entrez Gene: 379314 Xenopus laevis
- Omim: 600548 Human
- SwissProt: Q3ZCH0 Cow
see all -
别名
- 75 kDa glucose regulated protein antibody
- 75 kDa glucose-regulated protein antibody
- CSA antibody
see all
图片
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All lanes : Anti-Grp75/MOT antibody (ab227215) at 1/2500 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 74 kDa7.5% SDS-PAGE gel.
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Paraffin-embedded rat duodenum tissue stained for Grp75/MOT using ab227215 at 1/500 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence of HeLa cells staining Grp75/MOT with ab227215 (1/500). Hoechst 33342 staining. (blue).
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Anti-Grp75/MOT antibody (ab227215) at 1/500 dilution + SCC-15 (Human squamous cell carcinoma, tongue) whole cell extract at 6 µg
Predicted band size: 74 kDa7.5% SDS-PAGE gel.
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Paraffin-embedded mouse duodenum tissue stained for Grp75/MOT using ab227215 at 1/500 dilution in immunohistochemical analysis.
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Paraffin-embedded rat brain tissue stained for Grp75/MOT using ab227215 at 1/500 dilution in immunohistochemical analysis.
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Grp75/MOT was immunoprecipitated from HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract with 5 µg of ab227215. Western blot was performed from the immunoprecipitate using ab227215. Anti-Rabbit IgG was used as secondary antibody.
Lane 1: Control IgG instead of ab227215 in HepG2 whole cell extract.
Lane 2: ab227215 IP in HepG2 whole cell extract.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab227215 尚未被引用在任何文献中。